Caspase-2, human recombinant

• Catalog number
  1082-25
• Price
  Please ask
• Size
  25 units

• Synonyms
  Caspase-2
• Alternative_names
  Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1
• Description
  A cysteine protease involved in apoptosis
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  10,000 – 13,000 units/mg
• Unit Definition
  One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
• Molecular Weight
  two large (19 kDa) and two small (12 kDa) subunits
• Storage Temp
  -70°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized powder
• Reconstitution Instructions
  Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
• Background Information
  Caspase-2 (also know as Ich-1, Nedd-2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis pro-caspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed active caspase-2 is a heterotetramer consisting of two large (19 kDa) and two small (12 kDa) subunits.The recombinant active human caspase-2 was expressed in E. coli. The active caspase-2 preferentially cleaves caspase-2 substrates (e.g., VDVAD-AFC or VDVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VDVAD-pNA (Cat. #1072-200) or Ac-VDVAD-AFC (1071-200).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Caspase-2  
• Gene symbol
  CASP4, CASP1P2, CASP10
• Short name
  Caspase-2, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  caspase-2, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1505
• Gene
  CASP4
• Long gene name
  caspase 4
• Synonyms gene name
  • caspase 4, apoptosis-related cysteine protease
  • caspase 4, apoptosis-related cysteine peptidase
• Synonyms
  • ICE(rel)II
  • ICH-2
  • TX
• GenBank acession
  • U25804
• Locus
  11q22.3
• Discovery year
  1996-07-22
• Entrez gene record
  837
• Pubmed identfication
  • 7797510
  • 9250871
• RefSeq identity
  • NM_001225
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000166078

Gene info

• Identity
  HGNC:43776
• Gene
  CASP1P2
• Long gene name
  caspase 1 pseudogene 2
• Synonyms gene name
  • caspase 1, apoptosis-related cysteine peptidase pseudogene 2
• Locus
  11q22.3
• Discovery year
  2012-02-24
• Entrez gene record
  440067
• RefSeq identity
  • NR_131905
• VEGA ID
  OTTHUMG00000166162

Gene info

• Identity
  HGNC:1500
• Gene
  CASP10
• Long gene name
  caspase 10
• Synonyms gene name
  • caspase 10, apoptosis-related cysteine protease
  • caspase 10, apoptosis-related cysteine peptidase
• Synonyms
  • MCH4
  • FLICE-2
• Synonyms name
  • FAS-associated death domain protein interleukin-1B-converting enzyme 2
• GenBank acession
  • U60519
• Locus
  2q33.1
• Discovery year
  1997-04-21
• Entrez gene record
  843
• Pubmed identfication
  • 8755496
• RefSeq identity
  • NM_032977
• Classification
  • Caspases
  • Death effector domain containing
  • Death inducing signaling complex
• VEGA ID
  OTTHUMG00000132818
• Locus Specific Databases
  • CASP10base: Mutation registry for Autoimmune lymphoproliferative syndrome, type II (ALPS2)
  • LRG_33

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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