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Human Recombinant HSP70 Protein

  • Catalog number
    SPR-115C
  • Product name
    Human Recombinant HSP70 Protein
  • Size
    2x100 µg
  • Stock availability
    In Stock
  • Scientific context
    Scientific_context
  • Protein target
    HSP70
  • Protein reactivity
    Human
  • Certificate of analysis
    This product has been certified >90% pure using SDS-PAGE analysis. The protein tested positive for ATPase activity using a Malachite Green assay.
  • Protein description
    Active Human Recombinant HSP70 Protein
  • Other name
    HSP70 1 Protein, HSP70 2 Protein, HSP70.1 Protein, HSP72 Protein, HSP73 Protein, HSPA1 Protein, HSPA1A Protein, HSPA1B Protein
  • Primary research area
    Cancer, Heat Shock
  • Category
    Protein
  • Brand name
    none
  • Origin
    Recombinant
  • NCBI number
    NM_005345
  • Gene number
    3303
  • Protein number
    P08107
  • Verified applications
    WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA
  • Relevant bio activity
    ATPase active
  • Protein expression model
    Baculovirus/Hi5 cells
  • Peptide sequence
    MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD
  • Protein purification
    Affinity Purified | Endotoxin-free
  • Purity pourcentage
    >90% High purity
  • Recommended buffer for storage
    20mM Tris, 150mM NaCl, 10% glycerol
  • Protein concentration
    Lot/batch specific. See included datasheet.
  • Protein specificity
    ~70 kDa
  • Protein tag
    His tag
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Supplementary useful information
    Please see included datasheet or contact us
  • Protein cell localization
    Cytoplasm
  • Bibliography
    1. Zho J. (1998) Cell. 94: 471-480. 2. Boorstein, W. R., Ziegelhoffer, T. & Craig, E. A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al., (1993) Mol. Cell. Biol. 13(2): 869-876. 8. Prapapanich V., et al., (1996) Mol. Cell. Biol. 16(11): 6200-6207. 9. Fernandez-Funez et al., (2000) Nature. 408(6808): 101-106.
  • Release date
    1-Sep-2007
  • PubMed number
    28026090, 22535471, 19953352
  • Tested applications
    Western Blot, Western Blot Control, Western Blot Control
  • Tested species reactivity
    Human
  • Representative figure
    Representative_figure
  • Representative figure legend
    SDS-Page of endotoxin-free his-tagged 70kDa human Hsp70 protein (SPR-115). SDS-Page of human HSP70 Protein (SPR-115)
  • Warnings
    Non-hazardous materials
  • Protein origin
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Other related products
    Other_related_products
  • Gene target
    HSP70 Protein
    • Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    HSPA1B, HSPA14, HSPA1A, HSPA1L
  • Short name
    Recombinant HSP70 Protein
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative technique
    rec