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Rat Anti-Mouse LAMP2 Monoclonal IgG1, Clone GL2A7

  • Catalog number
    SMC-141D-RPE
  • Product name
    Rat Anti-Mouse LAMP2 Monoclonal IgG1, Clone GL2A7
  • Size
    100 µg
  • Label
    RPE
  • Clone
    GL2A7
  • Immunogen
    Purified preparation of mouse liver lysosomal membranes
  • Antibody s full description
    Rat Anti-Mouse LAMP2 Monoclonal IgG1 Antibody, Clone: GL2A7: RPE
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    CD107b Antibody, Igp110 Antibody, Igp2 Antibody, Lamp2C Antibody, LampB Antibody, MAC3 Antibody, Lysosome-associated membrane glycoprotein 2 Antibody, LAMP-2 Antibody, Lysosome-associated membrane protein 2 Antibody, CD107 antigen-like family member B Antibody, Lysosomal membrane glycoprotein type B Antibody, LGP-B Antibody
  • Raised in
    Rat
  • Isotype
    IgG1, IgG1
  • Antibody s target
    LAMP2
  • Primary research fields
    Cell Signaling, Organelle Markers, Trafficking, Chaperones, Neuroscience
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, IP
  • Antibody s reactivity
    Human, Mouse, Rabbit
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~100-110kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-141 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane , Endosome , Lysosome , Endosome membrane , Lysosome membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).
  • Bibliography
    1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043. 2. Furuta K., et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer J., et al. (1996) J Cell Biol. 132(4): 565-76. 4. Lichter-Konecki U., et al (1999) Differentiation 65(1): 43-58. 5. Grutzkau A., et al. (2004) Cytometry A. 61(1): 62-68. 6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19. 7. Tanaka Y., et al. (2000) Nature 406: 902-906.
  • Released date
    1-Nov-2008
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001017959.1
  • Gene number
    16784
  • Protein number
    P17047
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7 (SMC-141). Tissue: Corneal Endothelial Cell (CEC). Species: Rabbit. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody (SMC-141) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green). Courtesy of: Eunduck E.P. Kay, Doheny Eye Institute. Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7 (SMC-141). Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 µg). Lane 3: Mouse 3T3NIH lysate (10 µg). Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody (SMC-141) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa. Rat Anti-LAMP2 Antibody [GL2A7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rabbit Corneal Endothelial Cell (CEC) (SMC-141) Rat Anti-LAMP2 Antibody [GL2A7] used in Western Blot (WB) on Human, Mouse HEK293 and 3T3NIH cell lysates (SMC-141)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "lysosomal-associated membrane protein 2", "CD107b and LAMP-2 and LAMPB and LGP110", "LAMP2 and IDBG-84399 and ENSG00000005893 and 3920", "protein domain specific binding", "Plasma membranes", "Lamp2 and IDBG-140038 and ENSMUSG00000016534 and 16784", "LAMP2 and IDBG-629871 and ENSBTAG00000007399 and 529148" ]
  • Gene target
    LAMP2 Monoclonal IgG1 GL2A7
    • Gene info
    Locus Specific Databases:Global Variome shared LOVDLRG_749
  • Gene symbol
    LAMP2
  • Host
    Rat
  • isotype filter
    • IgG1
  • Short name
    Anti-Mouse LAMP2 Monoclonal IgG1, Clone GL2A7
  • label filter
    • RPE
  • Clone name
    GL2A7
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies