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Rat Anti-Mouse HSF2 Monoclonal IgG, Clone 3E2

  • Catalog number
    SMC-119D-PCP
  • Product name
    Rat Anti-Mouse HSF2 Monoclonal IgG, Clone 3E2
  • Size
    100 µg
  • Label
    PerCP
  • Clone
    3E2
  • Immunogen
    Purified recombinant mouse HSF2 protein
  • Antibody s full description
    Rat Anti-Mouse HSF2 Monoclonal IgG Antibody, Clone: 3E2: PerCP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HSTF2 Antibody, Heat shock factor protein 2 Antibody, Heat shock transcription factor 2 Antibody, HSF 2 Antibody
  • Raised in
    Rat
  • Isotype
    IgG, IgG
  • Antibody s target
    HSF2
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Epigenetics
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, AM
  • Antibody s reactivity
    Human, Mouse, Rat, Guinea Pig (Cavia porcellus), Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig
  • Antibody s dilutions
    WB (1:250), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~69kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    4 µg/ml of SMC-119 was sufficient for detection of HSF2 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
  • Bibliography
    1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3.Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774. 7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334- 1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
  • Released date
    1-Nov-2007
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001129036.1
  • Gene number
    15500
  • Protein number
    P38533 
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (SMC-119). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (SMC-119) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (SMC-119). Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (SMC-119) at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland. Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (SMC-119). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (SMC-119) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Rat Anti-HSF2 Antibody [3E2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-119) Rat Anti-HSF2 Antibody [3E2] used in Western Blot (WB) on Human K562 cell lysates (SMC-119) Rat Anti-HSF2 Antibody [3E2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-119)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    HSF2 Monoclonal 3E2
    • Gene info
  • Gene symbol
    HSF2
  • Host
    Rat
  • isotype filter
    • IgG
  • Short name
    Anti-Mouse HSF2 Monoclonal IgG, Clone 3E2
  • label filter
    • PerCP
  • Clone name
    3E2
  • technique filter
    • anti-
    • Mouse
    • anti
    • IgG
  • Technique
    anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies, immunoglobulins