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Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa, Clone 25F2.D10

  • Catalog number
    SMC-173D-A700
  • Product name
    Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa, Clone 25F2.D10
  • Size
    100 µg
  • Label
    ATTO 700
  • Clone
    25F2.D10
  • Immunogen
    Mouse Aha1
  • Antibody s full description
    Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa Antibody, Clone: 25F2.D10: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody
  • Raised in
    Rat
  • Isotype
    IgG2a Kappa, IgG2a
  • Antibody s target
    AHA1
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, ELISA
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP ( 1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
  • Antibody s concentration
    1.59 mg/ml
  • Antibody s specificity
    Detects ~38kDa. Can run up to 45kDa on SDS Page.
  • Storage recommendations
    4ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-173 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Endoplasmic Reticulum
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
  • Bibliography
    1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
  • Released date
    1-Jan-2010
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_666148.1
  • Gene number
    217737
  • Protein number
    Q8BK64
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green). Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:1000. Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle. Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (SMC-173). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (SMC-173) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Rat Anti-Aha1 Antibody [25F2.D10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa cells (SMC-173) Rat Anti-Aha1 Antibody [25F2.D10] used in Immunoprecipitation (IP) on Human HeLa cells (SMC-173) Rat Anti-Aha1 Antibody [25F2.D10] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-173) Rat Anti-Aha1 Antibody [25F2.D10] used in Western Blot (WB) on Human Cell lysates (SMC-173)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    AHA1 Monoclonal IgG2a Kappa 25F2 D10
    • Gene info
    Gene info
  • Gene symbol
    AHSA1, AHSA2P
  • Host
    Rat
  • isotype filter
    • IgG2a Kappa
  • Short name
    Anti-Mouse AHA1 Monoclonal IgG2a Kappa, Clone 25F2 D10
  • label filter
    • ATTO 700
  • Clone name
    25F2
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies