- Catalog numberSPC-313D
- Product nameRabbit Anti-Salmon HSP70 (Fish) Polyclonal: Unconjugated
- Size100 µl
- PriceAsk For Price
- LabelUnconjugated
- ImmunogenSynthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid HSP70. The target is not present in any other HSP70 proteins.
- Antibody s targetSalmon HSP70
- Antibody s full descriptionRabbit Anti-Salmon HSP70 (Fish) Polyclonal
- Primary research fieldsCancer, Heat Shock
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameHSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
- Verified applicationsWB
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivitySalmon, Brook Trout (Salvelinus fontinalis), Rainbow Trout (Oncorhynchus mykiss), Three-spined stickleback (Gasterosteus aculeatus), Fish
- Antibody s recommended dilutions for useWB (1:5000); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromRabbit Antiserum
- Recommended buffer for storageLyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
- Antibody s concentrationSee included datasheet or contact our support service
- Antibody s specificityDetects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis0.2 µl/ml of SPC-313 was sufficient for detection of HSP70 in 20 µg of heat shocked (25°C) rainbow trout cytoplasmic fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm
- Tissue specificitySee included datasheet or contact our support service.
- Scientific context
- Bibliography1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 2. Rothman J. (1989) Cell. 59: 591-601. 3. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
- Released date1/Jan/2009
- NCBI numberNP_001135156.1
- Gene number100196655
- Protein numberB5DG30
- PubMed number27872197|26123780|22579662|21322548
- Tested applications|Western Blot|Western Blot|Western Blot
- Tested reactivityRainbow Trout|Gasterosteus aculeatus (Three-spined stickleback)|Trout|Trout
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendWestern blot analysis of Rainbow trout Tissue lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-313). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-313) at 1:5000. Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Rainbow trout Tissue lysates (SPC-313)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetSalmon HSP70 Fish Unconjugated
- Identity:HGNC:5233
- Gene:HSPA1B
- Long gene name:heat shock protein family A (Hsp70) member 1B
- Synonyms gene name:heat shock 70kD protein 1B, heat shock 70kDa protein 1B
- Synonyms:HSP70-2,
- Discovery year:1995-08-11
- Entrez gene record:3304
- Pubmed identification:1700760
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031202
- Identity:HGNC:5232
- Gene:HSPA1A
- Long gene name:heat shock protein family A (Hsp70) member 1A
- Synonyms gene name:heat shock 70kD protein 1A, heat shock 70kDa protein 1A
- Synonyms:HSP70-1,
- Discovery year:2001-06-22
- Entrez gene record:3303
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031201
- Identity:HGNC:29526
- Gene:HSPA14
- Long gene name:heat shock protein family A (Hsp70) member 14
- Synonyms gene name:heat shock 70kDa protein 14
- Synonyms:HSP70-4HSP70L1
- Discovery year:2004-04-20
- Entrez gene record:51182
- Pubmed identification:21208456
- RefSeq identity:NM_016299
- Classification:Heat shock 70kDa proteins
- Identity:HGNC:5234
- Gene:HSPA1L
- Long gene name:heat shock protein family A (Hsp70) member 1 like
- Synonyms gene name:heat shock 70kD protein-like 1, heat shock 70kDa protein 1-like
- Synonyms:HSP70-HOM, hum70t
- Discovery year:1989-05-12
- Entrez gene record:3305
- Pubmed identification:9685725, 9349405
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031208
- Gene symbolHSPA1B, HSPA1A, HSPA14, HSPA1L
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti-Salmon HSP70 (Fish) Polyclonal: Unconjugated
- label filter
- Unconjugated
- technique filter
- Polyclonal
- Rabbit
- anti-
- anti
- TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies