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Rabbit Anti-Rat PDI Polyclonal: ATTO 488

  • Catalog number
    SPC-114D-A488
  • Product name
    Rabbit Anti-Rat PDI Polyclonal: ATTO 488
  • Size
    100 µl
  • Label
    ATTO 488
  • Immunogen
    Rat PDI synthetic peptide conjugated to KLH
  • Antibody s target
    Rat PDI
  • Antibody s full description
    Rabbit Anti-Rat PDI Polyclonal
  • Primary research fields
    Cell Signaling, Trafficking, Chaperones, Organelle Markers
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    PDA2 Antibody, PDI Antibody, PDIA2 Antibody, PDIP Antibody, pancreatic protein disulfide isomerase Antibody
  • Verified applications
    WB, IHC, ICC/IF, IP
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Dog, Hamster, Monkey, Guinea Pig (Cavia porcellus), Sheep, Pig, African clawed frog (Xenopus laevis), Mollusk, Mussel (Perna viridis)
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~58kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-114 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
  • Antibody in cell
    Endoplasmic Reticulum, Endoplasmic Reticulum Lumen
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
  • Bibliography
    1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7. 2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5. 3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42. 4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95. 5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163. 6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819. 7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
  • Released date
    1/Jun/2008
  • NCBI number
    NP_001099245.2
  • Gene number
    287164
  • Protein number
    P04785
  • PubMed number
    25678563|25678563|21684241
  • Tested applications
    Western Blot|Western Blot|Western Blot
  • Tested reactivity
    Human|Mouse|Perna viridis (Asian green mussel)
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. | Western blot analysis of Rat tissue mix showing detection of PDI protein using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (SPC-114). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (SPC-114) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite. Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114) | Rabbit Anti-PDI Antibody used in Western blot (WB) on Rat tissue mix (SPC-114) | Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-114)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    PDI ATTO 488
    • Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    PDIA2, PADI1, ERP29, P4HB
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- PDI Polyclonal: ATTO 488
  • label filter
    • ATTO 488
  • technique filter
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies