- Catalog numberSPC-167D-RPE
- Product nameRabbit Anti-Rat GRP78 Polyclonal: RPE
- Size100 µg
- PriceAsk For Price
- LabelRPE
- ImmunogenHis-tagged rat GRP78
- Antibody s targetRat GRP78
- Antibody s full descriptionRabbit Anti-Rat GRP78 Polyclonal
- Primary research fieldsCancer, Heat Shock, Cell Signaling, Organelle Markers, Trafficking, Chaperones
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameBIP Antibody, Grp78 Antibody, HSPA5 Antibody, MIF2 Antibody, immunoglobulin heavy chain binding protein Antibody
- Verified applicationsWB, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~78kDa. Weak detection in human tissues.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis1 µg/ml of SPC-167 was sufficient for detection of Grp78 in 10 µg of rat brain cell lysate by colorimetric immunoblot analysis using Goat anti -rabbit IgG:HRP as the secondary antibody.
- Antibody in cellEndoplasmic Reticulum, Endoplasmic Reticulum Membrane, Melanosome
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextGRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti¬tumor drugs (6, 7).
- Bibliography1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.
- Released date27/Mar/2013
- NCBI numberNP_037215.1
- Gene number25617
- Protein numberP06761.1
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Rat Brain cell lysates showing detection of ~78 kDa GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-167) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~78 kDa. Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-167) | Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-167) | Rabbit Anti-GRP78 Antibody used in Western blot (WB) on Brain cell lysates (SPC-167)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetGRP78 RPE
- Identity:HGNC:10293
- Gene:RPE
- Long gene name:ribulose-5-phosphate-3-epimerase
- Discovery year:2001-06-22
- Entrez gene record:6120
- RefSeq identity:NM_006916
- VEGA ID:OTTHUMG00000154690
- Identity:HGNC:5238
- Gene:HSPA5
- Long gene name:heat shock protein family A (Hsp70) member 5
- Synonyms gene name:heat shock 70kD protein 5 (glucose-regulated protein, 78kD), heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
- Synonyms:BiP,
- Discovery year:1991-07-26
- Entrez gene record:3309
- Classification:Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000020672
- Gene symbolRPE, HSPA5
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- GRP78 Polyclonal: RPE
- label filter
- RPE
- technique filter
- Polyclonal
- Rabbit
- anti-
- anti
- TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies