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Rabbit Anti-Rat Cytochrome P450 Reductase Polyclonal: ATTO 700

  • Catalog number
    SPC-210D-A700
  • Product name
    Rabbit Anti-Rat Cytochrome P450 Reductase Polyclonal: ATTO 700
  • Size
    100 µg
  • Label
    ATTO 700
  • Immunogen
    Rat native full-length Cytochrome P450 Reductase purified from liver tissue
  • Antibody s target
    Rat Cytochrome P450 Reductase
  • Antibody s full description
    Rabbit Anti-Rat Cytochrome P450 Reductase Polyclonal
  • Primary research fields
    Cancer, Heat Shock, Cardiovascular System
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Cytochrome C Reductase Antibody, CCR Antibody, CPR Antibody, P450R Antibody, CYPOR Antibody, DKFZp686G04235 Antibody, FLJ26468 Antibody, NADPH Cytochrome Antibody, P450 Reductase Antibody, NADPH dependent cytochrome P450 reductase Antibody, NADPH-cytochrome P450 reductase Antibody, P450 (cytochrome) oxidoreductase Antibody, P450 Cytochrome Oxidoreductase Antibody, P450R Antibody, POR Antibody
  • Verified applications
    WB, IHC, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~77kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1 µg/ml of SPC-210 was sufficient for detection of Cytochrome P450 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Microsome
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Cytochrome P450 Reductase is an enyme that is required for the transfer of electrons from NADPH to cytochrome P450 in microsomes. It has also been found to transfer electrons to Heme oxygenase and cytochrome B5. Cytochrome P450 Reductase is found localized to the endoplasmic reticulum membrane, tethered by its hydrophobic N-terminal tail. Mutations in the gene encoding Cytochrome P450 Reductase (POR) have been found to cause congenital adrenal hyperplasia, Antley-Bixler syndrome, amenorrhea and disordered steroidogenesis.
  • Bibliography
    1. Pandey A.V. and Flück C.E. (2013). Pharmacology & therapeutics 138 (2): 229–54. 2. Jensen K. and Møller BL (2010). Phytochemistry 71 (2-3): 132–41. 3. Flück C.E. et al. (2004). Nature Genetics 36 (3): 228–230.
  • Released date
    7/May/2014
  • NCBI number
    NP_113764.1
  • Gene number
    29441
  • Protein number
    P00388
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:150 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite. | Western blot analysis of Human, Mouse, Rat Human, Mouse and Rat Lysates showing detection of ~ 75 kDa Cytochrome P450 Reductase protein using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Lane 1: MW ladder. Lane 2: Human lysate,  A431. Lane 3: Mouse lung lysate.  Lane 4: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:2000 for 1 hour at RT. Color Development: TMB solution for 11 min at RT. Predicted/Observed Size: ~ 75 kDa. Other Band(s): ~30 -250 in Rat lysate only. Rabbit Anti-Cytochrome P450 Reductase Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-210) | Rabbit Anti-Cytochrome P450 Reductase Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-210) | Rabbit Anti-Cytochrome P450 Reductase Antibody used in Western blot (WB) on Human, Mouse, Rat Human, Mouse and Rat Lysates (SPC-210)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Cytochrome P450 Reductase ATTO 700
    • Gene info
    Locus Specific Databases:PharmVar Human Cytochrome P450 (CYP) Allele Nomenclature
    Gene info
    Gene info
    Locus Specific Databases:PharmVar Human Cytochrome P450 (CYP) Allele NomenclatureLRG_1262
    Gene info
    Gene info
    Locus Specific Databases:PharmVar Human Cytochrome P450 (CYP) Allele NomenclatureALSOD, the Amyotrophic Lateral Sclerosis Online Genetic DatabaseLRG_303
  • Gene symbol
    CYP3A7, CYP24A1, CYP1A1, CYP51A1, CYP2D6
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- Cytochrome P450 Reductase Polyclonal: ATTO 700
  • label filter
    • ATTO 700
  • technique filter
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies