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Rabbit Anti-Rat Aquaporin 4 Polyclonal: ATTO 390

  • Catalog number
    SPC-505D-A390
  • Product name
    Rabbit Anti-Rat Aquaporin 4 Polyclonal: ATTO 390
  • Size
    100 µg
  • Label
    ATTO 390
  • Immunogen
    Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
  • Antibody s target
    Rat Aquaporin 4
  • Antibody s full description
    Rabbit Anti-Rat Aquaporin 4 Polyclonal
  • Primary research fields
    Neuroscience, Ion Channels, Aquaporins
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    AQP 4 Antibody, AQP-4 Antibody, AQP4 Antibody, AQP4_HUMAN Antibody, Aquaporin type 4 Antibody, Aquaporin-4 Antibody, Aquaporin4 Antibody, HMIWC 2 Antibody, HMIWC2 Antibody, Mercurial insensitive water channel Antibody, Mercurial-insensitive water channel Antibody, MGC22454 Antibody, MIWC Antibody, WCH 4 Antibody, WCH4 Antibody
  • Verified applications
    WB, IHC, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~35kDa. May detect larger glycosylated bands ~50kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6).
  • Bibliography
    1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Agre P., Nielsen S. (1996) Nephrologie. 17(7): 409-415. 4. Nagelhus E.A., Mathiisen T.M., Ottersen O.P. (2004) Neuroscience. 129(4): 905-913. 5. Lennon V.A., Kryzer T.J., Pittock S.J., Verkman A.S., Hinson S.R. (2005) J Exp Med. 202(4): 473-477. 6. Bradl M., Lassmann D.H. (2008) Int. MS Journal/MS Forum. 15(3): 75-78.
  • Released date
    6/Dec/2011
  • NCBI number
    NP_036957.1
  • Gene number
    25293
  • Protein number
    P47863
  • PubMed number
    27413198
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505). Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody (SPC-505) at 1:2000. Rabbit Anti-Aquaporin 4 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-505) | Rabbit Anti-Aquaporin 4 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-505)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Aquaporin 4 ATTO 390
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- Aquaporin 4 Polyclonal: ATTO 390
  • label filter
    • ATTO 390
  • technique filter
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies