gentaur logogentaur logo
x

Rabbit Anti-Plant HSP101/ClpB CT (Maize) Polyclonal: Unconjugated

  • Catalog number
    SPC-306B
  • Product name
    Rabbit Anti-Plant HSP101/ClpB CT (Maize) Polyclonal: Unconjugated
  • Size
    200 µl
  • Label
    Unconjugated
  • Immunogen
    15aa peptide sequence from the C-terminus of maize HSP101. Gene accession number AF077337.
  • Antibody s target
    Plant HSP101
  • Antibody s full description
    Rabbit Anti-Plant HSP101/ClpB CT (Maize) Polyclonal
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    heat shock protein 1010 Antibody, HOT1 Antibody, HSP101 Antibody, HSPS Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Plant, Maize (Zea mays)
  • Antibody s recommended dilutions for use
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Rabbit Antiserum
  • Recommended buffer for storage
    Lyophilized rabbit Antiserum. For reconstitution add 200 µl of sterile water.
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects the C-terminal domain of HSP101 ~101kDa. No reactivity to other cereal HSP101.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.7 µl/ml of SPC-306 was sufficient for detection of HSP101 in 30 µg of mature embryo maize extracts by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    HSPs/chaperones are known to be expressed in plants not only when they experience high temperatures stress, but also in response to a wide range of other environmental stresses such as water stress, salt stress, cold stress and oxidative stress (2, 3). HSP100 is one such major class that acts as a chaperone. HSP101/ClpB is a member of this HSP100 family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones. In spite of expression during heat stress, members of HSP100 protein family are also expressed during seed development. HSP101 protein is both nuclear and cytoplasmic-localized (1, 4).
  • Bibliography
    1. Nieto-Sotelo J., et al. (2002) Plant Cell. 14: 1621-1633. 2. Vierling E. (1991) Annu Rev. Plabt Physiol. Plant Mol. Biol. 42: 579-620. 3. Waters W., et al. (2004) Trends Plant Sci. 9: 244-252. 4. Nieto-Sotelo J., Kannan K.B., Martinex L.M. and Segal C. (1999) Gene. 230: 187-195.
  • Released date
    1/Jan/2009
  • NCBI number
    NP_001104935.1
  • Gene number
    541780
  • Protein number
    Q9S822
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western blot analysis of Maize Tissue lysates showing detection of HSP101 protein using Rabbit Anti-HSP101 Polyclonal Antibody (SPC-306). Primary Antibody: Rabbit Anti-HSP101 Polyclonal Antibody (SPC-306) at 1:2000 for 1 hour at RT. Secondary Antibody: HRP-conjugated Goat Anti-Rabbit at 1:10000. Rabbit Anti-HSP101 Antibody used in Western blot (WB) on Maize Tissue lysates (SPC-306)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    HSP101/ClpB CT Maize Unconjugated
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- HSP101/ClpB CT (Maize) Polyclonal: Unconjugated
  • label filter
    • Unconjugated
  • technique filter
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies