- Catalog numberSPC-186D-RPE
- Product nameRabbit Anti-P. falciparum HSP70 (P. falciparum) Polyclonal: RPE
- Size100 µg
- PriceAsk For Price
- LabelRPE
- ImmunogenHis-tagged and purified PfHSP70, C-terminus (AA 365-681)
- Antibody s targetP. falciparum HSP70
- Antibody s full descriptionRabbit Anti-P. falciparum HSP70 (P. falciparum) Polyclonal
- Primary research fieldsCancer, Heat Shock
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameHSP70_PLAFA Antibody, Cytoplasmic antigen 74.3 kDa protein Antibody
- Verified applicationsWB, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityPlasmodium falciparum
- Antibody s recommended dilutions for useWB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~ 70kDa. Specific to P. falciparum and does not cross-react to any protein from Human erythrocytes.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis0.15 µg/ml of SPC-186 was sufficient for detection of PfHSP70 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm
- Tissue specificitySee included datasheet or contact our support service.
- Scientific context
- Bibliography1. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1) 1-17. 2. Rothman J. (1989) Cell 59: 591 -601. 3. DeLuca-Flaherty, et al. (1990) Cell. 62: 875-887. 4. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 5. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 6. Pesce E.R., et al. (2008) Int J Biochem Cell Biol. 40(12): 2914-26. 7. Pavithra S.R, Banumathy G., Joy O., Singh V., Tatu U. (2004) J Biol Chem. 279(45): 46692-9.
- Released date27/Apr/2009
- NCBI numberM19753
- Gene numberRefer to GenBank
- Protein numberP11144
- PubMed number26147206|25751816|23615908
- Tested applicationsImmunocytochemistry/Immunofluorescence|Western Blot|Immunocytochemistry/Immunofluorescence
- Tested reactivityP. falciparum|P. falciparum|P. falciparum
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendWestern blot analysis of Parasite Lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-186). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-186) at 1:2000. Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Parasite Lysates (SPC-186)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetP falciparum HSP70 P falciparum RPE
- Identity:HGNC:10293
- Gene:RPE
- Long gene name:ribulose-5-phosphate-3-epimerase
- Discovery year:2001-06-22
- Entrez gene record:6120
- RefSeq identity:NM_006916
- VEGA ID:OTTHUMG00000154690
- Identity:HGNC:5233
- Gene:HSPA1B
- Long gene name:heat shock protein family A (Hsp70) member 1B
- Synonyms gene name:heat shock 70kD protein 1B, heat shock 70kDa protein 1B
- Synonyms:HSP70-2,
- Discovery year:1995-08-11
- Entrez gene record:3304
- Pubmed identification:1700760
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031202
- Identity:HGNC:5232
- Gene:HSPA1A
- Long gene name:heat shock protein family A (Hsp70) member 1A
- Synonyms gene name:heat shock 70kD protein 1A, heat shock 70kDa protein 1A
- Synonyms:HSP70-1,
- Discovery year:2001-06-22
- Entrez gene record:3303
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031201
- Identity:HGNC:29526
- Gene:HSPA14
- Long gene name:heat shock protein family A (Hsp70) member 14
- Synonyms gene name:heat shock 70kDa protein 14
- Synonyms:HSP70-4HSP70L1
- Discovery year:2004-04-20
- Entrez gene record:51182
- Pubmed identification:21208456
- RefSeq identity:NM_016299
- Classification:Heat shock 70kDa proteins
- Identity:HGNC:5234
- Gene:HSPA1L
- Long gene name:heat shock protein family A (Hsp70) member 1 like
- Synonyms gene name:heat shock 70kD protein-like 1, heat shock 70kDa protein 1-like
- Synonyms:HSP70-HOM, hum70t
- Discovery year:1989-05-12
- Entrez gene record:3305
- Pubmed identification:9685725, 9349405
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031208
- Gene symbolRPE, HSPA1B, HSPA1A, HSPA14, HSPA1L
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti-P. falciparum HSP70 (P. falciparum) Polyclonal: RPE
- label filter
- RPE
- technique filter
- Polyclonal
- Rabbit
- anti-
- anti
- TechniquePolyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies