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Rabbit Anti-Human TNF alpha Polyclonal: ATTO 390

  • Catalog number
    SPC-1315D-A390
  • Product name
    Rabbit Anti-Human TNF alpha Polyclonal: ATTO 390
  • Size
    100 µl
  • Label
    ATTO 390
  • Immunogen
    Synthesized peptide derived from internal of human TNFA.
  • Antibody s target
    Human TNF alpha
  • Antibody s full description
    Rabbit Anti-Human TNF alpha Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    APC1 Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, Differentiation inducing factor Antibody, Macrophage cytotoxic factor Antibody, TNF alpha Antibody, Tnf Antibody, TNF superfamily, member 2 Antibody, TNF, macrophage derived Antibody, TNF, monocyte derived Antibody, TNF-a Antibody, TNF-alpha Antibody, TNFA Antibody, TNFA_HUMAN Antibody, TNFSF2 Antibody, Tumor necrosis factor alpha Antibody, Tumor necrosis factor Antibody, Tumor necrosis factor ligand superfamily member 2 Antibody, Tumor Necrosis Factor, Membrane Form Antibody, Tumor necrosis factor, soluble form Antibody
  • Verified applications
    IHC, ICC/IF, ELISA
  • Raised in
    Rabbit
  • Isotype
    IgG
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    IHC (1:100), ICC/IF (1:500), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects endogenous levels of total TNFA protein.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:100 dilution of SPC-1315 was sufficient for detection of TNF alpha in human HepG2 cells using Goat Anti-Rabbit IgG as the secondary antibody.
  • Antibody in cell
    Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Tumor necrosis factor is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD).
  • Bibliography
    1. Swardfager W et al. (2010) Biol Psychiatry 68 (10): 930–941. 2. Locksley RM et al. (2001) Cell 104 (4): 487–501. 3. Dowlati Y et al. (2010) Biol Psychiatry 67 (5): 446–457. 4. Victor FC and Gottlieb AB (2002) J Drugs Dermatol 1 (3): 264–75. 5. Brynskov J. (2002) Gut 51 (1): 37–43.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_000585.2
  • Gene number
    7124
  • Protein number
    P01375
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-TNF alpha Antibody used in Immunohistochemistry (IHC) on Colon Carcinoma Tissue (SPC-1315) | Rabbit Anti-TNF alpha Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HepG2 cells (SPC-1315)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "tumor necrosis factor", "DIF and TNF-alpha and TNFA and TNFSF2", "TNF and IDBG-300259 and ENSG00000232810 and 7124", "transcription regulatory region DNA binding", "Extracellular", "Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926", "TNF and IDBG-634161 and ENSBTAG00000025471 and 280943" ]
  • Gene target
    TNF alpha ATTO 390
    • Gene info
    Gene info
    Locus Specific Databases:INFEVERS: The repertory of Familial Mediterranean Fever (FMF) and Hereditary Inflammatory Disorders MutationsLRG_193
    Gene info
    Gene info
  • Gene symbol
    TNF, TNFRSF1A, TNFRSF1B, LTBR
  • Host
    Rabbit, Rabbits
  • isotype filter
    • IgG
  • Short name
    Rabbit Anti- TNF alpha Polyclonal: ATTO 390
  • label filter
    • ATTO 390
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies