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Rabbit Anti-Human SOD1 (EDI) Polyclonal: ATTO 633

  • Catalog number
    SPC-206D-A633
  • Product name
    Rabbit Anti-Human SOD1 (EDI) Polyclonal: ATTO 633
  • Size
    100 µg
  • Label
    ATTO 633
  • Immunogen
    N-terminal region of SOD1, exposed dimer interface (EDI)
  • Antibody s target
    Human SOD1
  • Antibody s full description
    Rabbit Anti-Human SOD1 (EDI) Polyclonal
  • Primary research fields
    Cancer, Oxidative Stress, Neuroscience, Neurodegeneration, ALS Disease
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    SEDI Antibody, SOD1 EDI Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 exposed dimer interface Antibody
  • Verified applications
    WB, ELISA
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Recognizes a conformation specific epitope where the dimer interface is exposed.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1µg/ml of SPC-206 was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1.
  • Bibliography
    1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 2. Furukawa Y., and O’Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67. 3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925. 4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.
  • Released date
    25/Sep/2013
  • NCBI number
    CAG46542
  • Gene number
    6647
  • Protein number
    P00441
  • PubMed number
    28057013
  • Tested applications
    ELISA
  • Tested reactivity
    Human
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western blot analysis of Mouse Lung showing detection of ~18 kDa SOD1 (EDI) protein using Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206) at 1:1000. Predicted/Observed Size: ~18 kDa. Rabbit Anti-SOD1 (EDI) Antibody used in Western blot (WB) on Mouse Lung (SPC-206)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "superoxide dismutase 1, soluble", "ALS and ALS1 and HEL-S-44 and homodimer and hSod1 and IPOA and SOD", "SOD1 and IDBG-1609 and ENSG00000142168 and 6647", "chaperone binding", "nuclei", "Sod1 and IDBG-174662 and ENSMUSG00000022982 and 20655" ]
  • Gene target
    SOD1 EDI ATTO 633
    • Gene info
    • Identity:HGNC:55683
    • Gene:SOD1-DT
    • Long gene name:SOD1 divergent transcript
    • Discovery year:2021-07-01
    • Entrez gene record:102724449
    • Classification:Divergent transcripts
    Gene info
    Locus Specific Databases:ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic DatabaseLRG_652
  • Gene symbol
    SOD1-DT, SOD1
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- SOD1 (EDI) Polyclonal: ATTO 633
  • label filter
    • ATTO 633
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies