- Catalog numberSPC-173D-P594
- Product nameRabbit Anti-Human RAS Polyclonal: PE/ATTO 594
- Size100 µg
- PriceAsk For Price
- LabelPE/ATTO 594
- ImmunogenSynthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
- Antibody s targetHuman RAS
- Antibody s full descriptionRabbit Anti-Human RAS Polyclonal
- Primary research fieldsCancer, Cell Signaling, Epigenetics
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameGTPase Hras Antibody, GTPase Kras Antibody, GTPase Nras Antibody, Ha Ras Antibody, K Ras Antibody, K ras p21 Antibody, KRAS Antibody, NRAS Antibody, p21ras Antibody, RASH2 Antibody
- Verified applicationsWB, IHC, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat, Bovine
- Antibody s recommended dilutions for useWB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein G Purified
- Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~21kDa.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellCell Membrane, Golgi Apparatus, Golgi Apparatus Membrane
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextThe 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
- Bibliography1. Boguski M.S. and McCormick F. (1993) Nature 366: 643–654. 2. Avruch J., et al. (1994) Trends Biochem. Sci.19: 279–283. 3. Buday L. and Downward J. (1993) Cell 73: 611–620. 4. Huang D.C., et al. (1993) Mol. Cell Biol. 13: 2420–2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682–4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114–131.
- Released date26/Jul/2012
- NCBI numberNP_035067.2
- Gene number18176
- Protein numberB2KGV5
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:200 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis. | Western blot analysis of Mouse Brain Cortex cell lysates showing detection of ~21 kDa RAS protein using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Cortex cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite. Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-173) | Rabbit Anti-RAS Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-173) | Rabbit Anti-RAS Antibody used in Western blot (WB) on Brain Cortex cell lysates (SPC-173) | Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-173) | Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-173)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetRAS PE/ATTO 594
- Identity:HGNC:7989
- Gene:NRAS
- Long gene name:NRAS proto-oncogene, GTPase
- Synonyms gene name:neuroblastoma RAS viral (v-ras) oncogene homolog, neuroblastoma RAS viral oncogene homolog
- Synonyms:N-ras,
- Discovery year:2001-06-22
- Entrez gene record:4893
- Pubmed identification:6705568, 11524732
- RefSeq identity:NM_002524
- Classification:RAS type GTPase family
- VEGA ID:OTTHUMG00000012059
- Identity:HGNC:10447
- Gene:RRAS
- Long gene name:RAS related
- Synonyms gene name:related RAS viral (r-ras) oncogene homolog
- Synonyms:R-Ras
- Discovery year:2001-06-22
- Entrez gene record:6237
- Pubmed identification:3098437
- RefSeq identity:NM_006270
- Classification:RAS type GTPase family
- VEGA ID:OTTHUMG00000183247
- Gene symbolNRAS, RRAS, RASGRF2, MRAS
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- RAS Polyclonal: PE/ATTO 594
- label filter
- PE ATTO 594
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies
Gene info
Locus Specific Databases:NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IVLRG_92