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Immunogen
Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
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Antibody s target
Human RAS
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Antibody s full description
Rabbit Anti-Human RAS Polyclonal
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Primary research fields
Cancer, Cell Signaling, Epigenetics
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
GTPase Hras Antibody, GTPase Kras Antibody, GTPase Nras Antibody, Ha Ras Antibody, K Ras Antibody, K ras p21 Antibody, KRAS Antibody, NRAS Antibody, p21ras Antibody, RASH2 Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat, Bovine
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein G Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~21kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Membrane, Golgi Apparatus, Golgi Apparatus Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
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Bibliography
1. Boguski M.S. and McCormick F. (1993) Nature 366: 643–654. 2. Avruch J., et al. (1994) Trends Biochem. Sci.19: 279–283. 3. Buday L. and Downward J. (1993) Cell 73: 611–620. 4. Huang D.C., et al. (1993) Mol. Cell Biol. 13: 2420–2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682–4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114–131.
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Released date
26/Jul/2012
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NCBI number
NP_035067.2
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Gene number
18176
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Protein number
B2KGV5
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:200 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite. | Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis. | Western blot analysis of Mouse Brain Cortex cell lysates showing detection of ~21 kDa RAS protein using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Cortex cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (SPC-173). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (SPC-173) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite. Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-173) | Rabbit Anti-RAS Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-173) | Rabbit Anti-RAS Antibody used in Western blot (WB) on Brain Cortex cell lysates (SPC-173) | Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-173) | Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-173)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
NRAS, RRAS, RASGRF2, MRAS
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Short name
Rabbit Anti- RAS Polyclonal: PE/ATTO 594
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
PE/ATTO 594
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman RAS polyclonal: peroxidase/ATTO 594
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Alternative technique
polyclonals, rabbit-anti, antibodies
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