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Rabbit Anti-Human PUMA (CT) Polyclonal: FITC

  • Catalog number
    SPC-166D-FITC
  • Product name
    Rabbit Anti-Human PUMA (CT) Polyclonal: FITC
  • Size
    100 µg
  • Label
    FITC
  • Immunogen
    C-terminal amino acids of human PUMA
  • Antibody s target
    Human PUMA
  • Antibody s full description
    Rabbit Anti-Human PUMA (CT) Polyclonal
  • Primary research fields
    Cancer, Apoptosis
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 0.02% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
  • Bibliography
    1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_001120712.1
  • Gene number
    27116
  • Protein number
    Q9BXH4
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human, Mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:1000. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:100. Rabbit Anti-PUMA (CT) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-166) | Rabbit Anti-PUMA Antibody used in Western blot (WB) on Human, Mouse K562 and 3T3 cell lysates (SPC-166) | Rabbit Anti-PUMA (CT) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-166) | Rabbit Anti-PUMA Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human K562 cells (SPC-166)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    PUMA CT
    • Gene info
    Gene info
  • Gene symbol
    BBC3, HCAR2
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- PUMA (CT) Polyclonal: FITC
  • label filter
    • FITC
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
    • FITC
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies, fluorescine