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Rabbit Anti-Human LC3B Polyclonal: ATTO 633

  • Catalog number
    SPC-217A-A633
  • Product name
    Rabbit Anti-Human LC3B Polyclonal: ATTO 633
  • Size
    50 µg
  • Label
    ATTO 633
  • Immunogen
    Peptide derived from C-terminal region of human LC3B protein
  • Antibody s target
    Human LC3B
  • Antibody s full description
    Rabbit Anti-Human LC3B Polyclonal
  • Primary research fields
    Cancer, Autophagy, Cell Signaling, Neuroscience, Cardiovascular System, Heart
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    MAP1LC3B Antibody, Autophagy related protein LC3 B Antibody, Autophagy related ubiquitin like modifier LC3 B Antibody, Autophagy-related protein LC3 B Antibody, Autophagy-related ubiquitin-like modifier LC3 B Antibody, LC3 Antibody, MAP1 light chain 3 like protein 2 Antibody, MAP1 light chain 3-like protein 2 Antibody, MAP1A/1B light chain 3 B Antibody, MAP1A/1BLC3 Antibody, Antibody, MAP1A/MAP1B LC3 B Antibody, MAP1A/MAP1B light chain 3 B Antibody, Antibody, MAP1BLC3 Antibody, Antibody, Map1lc3b Antibody, Antibody, Microtubule associated protein 1 light chain 3 beta Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody, Microtubule associated proteins 1A/1B light chain 3A Antibody, Microtubule associated proteins 1A/1B light chain 3B Antibody, Microtubule-associated protein 1 light chain 3 beta Antibody, Microtubule-associated proteins 1A/1B light chain 3B Antibody, MLP3B_HUMAN Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 15mM sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Specific for LC3B. Does not detect LC3A, LC3C, GABARAP, GABARAPL1 or GABARAPL2. Detects LC3B-I and LC3B-II.
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:10000 dilution of SPC-217 was sufficient for detection of LC3B in lysate from cells expressing Recombinant GST-tagged LC3B by electrochemiluminescence analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Cytoskeleton, Endomembrane System, Lipid-anchor, Cytoplasmic Vesicle, Autophagosome, Autophagosome Membrane
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
  • Bibliography
    1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
  • Released date
    12/Jun/2014
  • NCBI number
    NP_073729.6
  • Gene number
    81636
  • Protein number
    Q9GZQ13
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.05
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    LC3B ATTO 633
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- LC3B Polyclonal: ATTO 633
  • label filter
    • ATTO 633
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies