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Rabbit Anti-Human HSP27 (Ser78) Polyclonal: ATTO 633

  • Catalog number
    SPC-1261D-A633
  • Product name
    Rabbit Anti-Human HSP27 (Ser78) Polyclonal: ATTO 633
  • Size
    100 µl
  • Label
    ATTO 633
  • Immunogen
    Synthesized unphosphorylated peptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
  • Antibody s target
    Human HSP27 (Ser78)
  • Antibody s full description
    Rabbit Anti-Human HSP27 (Ser78) Polyclonal
  • Primary research fields
    Cancer, Heat Shock, Cardiovascular System, Atherosclerosis, Heart, Trafficking, Chaperones
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody
  • Verified applications
    WB, IHC
  • Raised in
    Rabbit
  • Isotype
    IgG
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects endogenous levels of total HSP27 protein.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1261 was sufficient for detection of Hsp27 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Nucleus
  • Tissue specificity
    Ubiquituous.
  • Scientific context
    Scientific_context
  • Bibliography
    1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_001531.1
  • Gene number
    3315
  • Protein number
    P04792
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1261). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1261) at 1:1000. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HeLa cell lysates showing detection of ~27kDa Hsp27 protein using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1261). Lane 1 and Lane 2: Human HeLa, Hsp27 Antibody (Ser78). Lane 3 and Lane 4: Human HeLa, Hsp27 Antibody (pSer78). Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1261) at 1:1000. Predicted/Observed Size: ~27kDa. Rabbit Anti-Hsp27 Antibody (Ser78) used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1261) | Rabbit Anti-Hsp27 Antibody (Ser78) used in Western blot (WB) on HeLa cell lysates (SPC-1261)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    HSP27 Ser78 ATTO 633
    • Gene info
    Locus Specific Databases:Inherited Peripheral Neuropathies Mutation DatabaseLRG_248
  • Gene symbol
    HSPB1
  • Host
    Rabbit, Rabbits
  • isotype filter
    • IgG
  • Short name
    Rabbit Anti- HSP27 (Ser78) Polyclonal: ATTO 633
  • label filter
    • ATTO 633
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies