- Catalog numberSPC-208D-A633
- Product nameRabbit Anti-Human HSF1 Polyclonal: ATTO 633
- Size100 µg
- PriceAsk For Price
- LabelATTO 633
- ImmunogenPurified human HSF1 protein NM_005526.2
- Antibody s targetHuman HSF1
- Antibody s full descriptionRabbit Anti-Human HSF1 Polyclonal
- Primary research fieldsCancer, Heat Shock, Cell Signaling, Epigenetics, Cardiovascular System, Heart
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameHeat shock factor 1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody, hsf1 Antibody, HSF1_HUMAN Antibody, HSTF 1 Antibody, HSTF1 Antibody
- Verified applicationsWB, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~85kDa (unstressed cell lysates), and ~95kDa (heat shocked cell lysates).
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis1 µg/ml of SPC-208 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
- Antibody in cellCytoplasm, Nucleus
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextHSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
- Bibliography1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3. Tanaka K.I., et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., et al. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774. 7. Fiorenza M.T., et al. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
- Released date25/Sep/2013
- NCBI numberNP_005517.1
- Gene number3297
- Protein numberQ00613
- PubMed number21167840
- Tested applicationsWestern Blot
- Tested reactivityMouse
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-HSF1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-208) | Rabbit Anti-HSF1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-208)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Alternative to gene target
- [ "heat shock transcription factor 1", "HSTF1", "HSF1 and IDBG-40498 and ENSG00000185122 and 3297", "sequence-specific DNA binding", "nuclei", "Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499", "HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235" ]
- Gene targetHSF1 ATTO 633
- Gene symbolHSF1
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- HSF1 Polyclonal: ATTO 633
- label filter
- ATTO 633
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies