- Catalog numberSPC-102D-FITC
- Product nameRabbit Anti-Human HSC70 (HSP73) Polyclonal: FITC
- Size100 µl
- PriceAsk For Price
- LabelFITC
- ImmunogenAmino acids 650-670 of human HSP73
- Antibody s targetHuman HSC70
- Antibody s full descriptionRabbit Anti-Human HSC70 (HSP73) Polyclonal
- Primary research fieldsCancer, Heat Shock
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameHSC54 Antibody, HSC71 Antibody, HSC73 Antibody, HSP71 Antibody, HSP73 Antibody, HSPA10 Antibody, HSPA8 Antibody, LAP1 Antibody, NIP71 Antibody
- Verified applicationsWB, IHC, ICC/IF, IP, Bl
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat, Hamster
- Antibody s recommended dilutions for useWB (1:2000), IHC (1:100), ICC/IF (1:250); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromRabbit Antiserum
- Recommended buffer for storageRabbit Antiserum
- Antibody s concentrationSee included datasheet or contact our support service
- Antibody s specificityDetects 73kDa. Does not cross-react with HSP70.
- Storage recommendations4°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis0.5 µg/ml of SPC-102 was sufficient for detection of HSC70 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm, Melanosome
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextHSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g. heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
- Bibliography1. Brown C.L. et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989), Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 6. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
- Released date1/Jun/2007
- NCBI numberNP_006588.1
- Gene number3312
- Protein numberP11142
- PubMed number24361285|24447452|24447452|24056538|23060796|20018937|19209902
- Tested applicationsImmunoprecipitation|Immunohistochemistry|Western Blot |Immunocytochemistry/Immunofluorescence|Western Blot|Western Blot|Western Blot
- Tested reactivityHuman|Human|Human|Human|Human|Human|Rat
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. | Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. | Western blot analysis of Human Cell line lysates showing detection of HSC70 protein using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSC70 Polyclonal Antibody (SPC-102) at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Rabbit Anti-HSC70 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-102) | Rabbit Anti-HSC70 Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-102) | Rabbit Anti-HSC70 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-102) | Rabbit Anti-HSC70 Antibody used in Immunohistochemistry (IHC) on Mouse Inflamed colon (SPC-102)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetHSC70 HSP73
- Identity:HGNC:5241
- Gene:HSPA8
- Long gene name:heat shock protein family A (Hsp70) member 8
- Synonyms gene name:heat shock 70kD protein 8, heat shock 70kDa protein 8
- Synonyms:HSC71, HSC70HSP73
- Discovery year:1995-05-08
- Entrez gene record:3312
- Pubmed identification:8530083, 3037489
- Classification:Spliceosomal P complex, Heat shock 70kDa proteins, Spliceosomal C complex
- VEGA ID:OTTHUMG00000166030
- Gene symbolHSPA8
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- HSC70 (HSP73) Polyclonal: FITC
- label filter
- FITC
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- FITC
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies, fluorescine