- Catalog numberSPC-112D-A680
- Product nameRabbit Anti-Human HO-1 Polyclonal: ATTO 680
- Size100 µg
- PriceAsk For Price
- LabelATTO 680
- ImmunogenHuman heme-oxygenase (HO-1) synthetic multiple antigenic peptide
- Antibody s targetHuman HO-1
- Antibody s full descriptionRabbit Anti-Human HO-1 Polyclonal
- Primary research fieldsCancer, Oxidative Stress
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameHeme oxygenase 1 Antibody, Hemox Antibody, HMOX1 Antibody, HO1 Antibody, HO 1 Antibody, HSP32 Antibody
- Verified applicationsWB, ICC/IF, IHC
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat, Dog
- Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~32kDa.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis1 µg/ml of SPC-112 was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellEndoplasmic Reticulum, Microsome
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextHeme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
- Bibliography1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.
- Released date1/Nov/2008
- NCBI numberNP_002124.1
- Gene number3162
- Protein numberP09601
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h. | Western blot analysis of Human Cell line lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody (SPC-112) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-112) | Rabbit Anti-HO-1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-112) | Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-112) | Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-112)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetHO-1 ATTO 680
- Identity:HGNC:5013
- Gene:HMOX1
- Long gene name:heme oxygenase 1
- Synonyms gene name:heme oxygenase (decycling) 1
- Synonyms:bK286B10HO-1
- Discovery year:1992-10-15
- Entrez gene record:3162
- Pubmed identification:10591208
- VEGA ID:OTTHUMG00000150960
- Identity:HGNC:5014
- Gene:HMOX2
- Long gene name:heme oxygenase 2
- Synonyms gene name:heme oxygenase (decycling) 2
- Synonyms:HO-2
- Discovery year:1992-10-15
- Entrez gene record:3163
- Pubmed identification:1575508
- VEGA ID:OTTHUMG00000129473
- Identity:HGNC:5670
- Gene:IGHVII-1-1
- Long gene name:immunoglobulin heavy variable (II)-1-1 (pseudogene)
- Synonyms gene name:immunoglobulin heavy variable (II)-1-1, immunoglobulin heavy variable (II)-1-1 pseudogene
- Synonyms:IGHV(II)-1-1,
- Discovery year:2000-04-17
- Entrez gene record:28377
- RefSeq identity:NG_001019
- Classification:Immunoglobulin heavy locus at 14q32.33
- VEGA ID:OTTHUMG00000152325
- Identity:HGNC:31499
- Gene:MIR1-1
- Long gene name:microRNA 1-1
- Synonyms:hsa-mir-1-1
- Discovery year:2004-04-23
- Entrez gene record:406904
- RefSeq identity:NR_029780
- Classification:MicroRNA MIR1/206 family
- Identity:HGNC:50820
- Gene:TRUND-NNN8-1
- Long gene name:tRNA-undetermined (NNN) 8-1
- Synonyms gene name:transfer RNA-undetermined (NNN) 8-1
- Discovery year:2014-06-20
- Entrez gene record:103482528
- Pubmed identification:18984615
- Identity:HGNC:50819
- Gene:TRUND-NNN7-1
- Long gene name:tRNA-undetermined (NNN) 7-1
- Synonyms gene name:transfer RNA-undetermined (NNN) 7-1
- Discovery year:2014-06-20
- Entrez gene record:103482531
- Pubmed identification:18984615
- Identity:HGNC:5760
- Gene:IGKV1OR2-1
- Long gene name:immunoglobulin kappa variable 1/OR2-1 (pseudogene)
- Synonyms gene name:immunoglobulin kappa variable 1/OR-1, immunoglobulin kappa variable 1/OR-1 pseudogene, immunoglobulin kappa variable 1/OR-1 (pseudogene)
- Synonyms:IGKV1/OR-1, IGKV1/OR2-1,
- Discovery year:2000-04-18
- Entrez gene record:3531
- Pubmed identification:2853098
- Classification:Immunoglobulin kappa (IGK) orphons
- VEGA ID:OTTHUMG00000155081
- Identity:HGNC:35281
- Gene:MIR1289-1
- Long gene name:microRNA 1289-1
- Synonyms:hsa-mir-1289-1
- Discovery year:2008-11-03
- Entrez gene record:100302125
- RefSeq identity:NR_031620
- Classification:MicroRNA MIR1289 family
- Identity:HGNC:31488
- Gene:MIR101-1
- Long gene name:microRNA 101-1
- Synonyms:hsa-mir-101-1
- Discovery year:2004-04-23
- Entrez gene record:406893
- RefSeq identity:NR_029516
- Classification:MicroRNA MIR101 family
- Identity:HGNC:31545
- Gene:MIR16-1
- Long gene name:microRNA 16-1
- Synonyms:hsa-mir-16-1
- Discovery year:2004-04-23
- Entrez gene record:406950
- RefSeq identity:NR_029486
- Classification:MicroRNA MIR15/16 family
- Gene symbolHMOX1, HMOX2, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- HO-1 Polyclonal: ATTO 680
- label filter
- ATTO 680
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies