gentaur logogentaur logo
x

Rabbit Anti-Human Histone H3 (pSer10) Polyclonal: ATTO 655

  • Catalog number
    SPC-1256D-A655
  • Product name
    Rabbit Anti-Human Histone H3 (pSer10) Polyclonal: ATTO 655
  • Size
    100 µl
  • Label
    ATTO 655
  • Immunogen
    A synthesized peptide derived from human Histone H3
  • Antibody s target
    Human Histone H3 (pSer10)
  • Antibody s full description
    Rabbit Anti-Human Histone H3 (pSer10) Polyclonal
  • Primary research fields
    Cell Signaling, Epigenetics and Nuclear Signalling, Histones
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Isotype
    IgG
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, pH 7.4, 50% glycerol, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects endogenous Histone H3(Phospho Ser10) protein.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1256 was sufficient for detection of Histone H3 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Nucleus, Chromosome
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
  • Bibliography
    1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_003520.1
  • Gene number
    8350
  • Protein number
    P68431/Q71DI3/P84243
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western blot analysis of Human HeLa cell lysates showing detection of ~15-17kDa Histone H3 protein using Rabbit Anti-Histone H3 Polyclonal Antibody (SPC-1256). Lane 1: Human HeLa untreated (-). Lane 2: Human HeLa treated with Nocodazole (1µg/ml, 24 hr). Primary Antibody: Rabbit Anti-Histone H3 Polyclonal Antibody (SPC-1256) at 1:1000. Predicted/Observed Size: ~15-17kDa. Rabbit Anti-Histone H3 Antibody (pSer10) used in Western blot (WB) on HeLa cell lysates (SPC-1256)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Histone H3 pSer10 ATTO 655
  • Host
    Rabbit, Rabbits
  • isotype filter
    • IgG
  • Short name
    Rabbit Anti- Histone H3 (pSer10) Polyclonal: ATTO 655
  • label filter
    • ATTO 655
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies