- Catalog numberSPC-1255D-A565
- Product nameRabbit Anti-Human Histone H3 (Acetyl pLys9) Polyclonal: ATTO 565
- Size100 µl
- PriceAsk For Price
- LabelATTO 565
- ImmunogenA synthesized peptide derived from human Acetyl-Histone H3 around the phosphorylation site of Lys9
- Antibody s targetHuman Histone H3 (Acetyl pLys9)
- Antibody s full descriptionRabbit Anti-Human Histone H3 (Acetyl pLys9) Polyclonal
- Primary research fieldsCell Signaling, Epigenetics and Nuclear Signalling, Histones
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameH3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
- Verified applicationsWB, IHC, ICC/IF
- Raised inRabbit
- IsotypeIgG
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s recommended dilutions for useWB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromPeptide Affinity Purified
- Recommended buffer for storagePBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
- Antibody s concentrationSee included datasheet or contact our support service
- Antibody s specificityDetects ~17kDa, endogenous levels of Acetyl-Histone H3 only when phosphorylated at Lys9
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysisA 1:1000 dilution of SPC-1255 was sufficient for detection of Histone H3 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
- Antibody in cellNucleus, Chromosome
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextHistone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
- Bibliography1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.
- Released date6/Sep/2016
- NCBI numberNP_003520.1/NP_066403.2/NP_002098.1
- Gene number8350/653604/3020
- Protein numberP68431/Q71DI3/P84243
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure linkNo Data Available
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetHistone H3 pLys9 ATTO 565
- HostRabbit, Rabbits
- isotype filter
- IgG
- Short nameRabbit Anti- Histone H3 ( pLys9) Polyclonal: ATTO 565
- label filter
- ATTO 565
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies