- Catalog numberSPC-622D-A565
- Product nameRabbit Anti-Human GABARAPL1 Polyclonal: ATTO 565
- Size100 µg
- PriceAsk For Price
- LabelATTO 565
- ImmunogenSynthetic peptide from the N-terminal of Human GABARAPL1 (aa. 5-16)
- Antibody s targetHuman GABARAPL1
- Antibody s full descriptionRabbit Anti-Human GABARAPL1 Polyclonal
- Primary research fieldsNeuroscience, Neurotransmitter Receptors, GABA Receptors
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameAPG8 like Antibody, GEC-1 Antibody, ATG8 Antibody, Glandular epithelial cell protein 1 Antibody, ATG8B Antibody, GEC1 Antibody, GBRL1_HUMAN Antibody, Gamma-aminobutyric acid receptor-associated protein-like 1 Antibody, GABARAPL1 a Antibody, ATG8L Antibody, APG8L Antibody, GABA(A) receptor-associated protein-like 1 Antibody, Early estrogen regulated protein Antibody, Early estrogen-regulated protein Antibody, GABA(A) receptor associated protein like 1 Antibody, Gamma aminobutyric acid receptor associated protein like 1 Antibody, GEC 1 Antibody, GABARAPL1 Antibody, GABA Antibody,
- Verified applicationsWB, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse
- Antibody s recommended dilutions for useWB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromPeptide Affinity Purified
- Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~18 kDa.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysisA 1:1000 dilution of SPC-622 was sufficient for detection of GABARAPL1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm, Cytoskeleton, Cytoplasmic Vesicle Membrane, Lipid-Anchor, Endoplasmic Reticulum, Golgi Apparatus, Cytoplasmic Vesicle, Autophagosome
- Tissue specificityUbiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
- Scientific contextSee included datasheet or contact our support service.
- BibliographyRefer to PubMed
- Released date29/Aug/2016
- NCBI numberNP_113600.1
- Gene number23710
- Protein numberQ9H0R8
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome, Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL1 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa. Rabbit Anti-GABARAPL1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-622) | Rabbit Anti-GABARAPL1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-622)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetGABARAPL1 ATTO 565
- Identity:HGNC:54822
- Gene:GABARAPL1-AS1
- Long gene name:GABARAPL1 antisense RNA 1
- Discovery year:2020-01-17
- Pubmed identification:32079618
- Classification:Antisense RNAs
- Identity:HGNC:4068
- Gene:GABARAPL1
- Long gene name:GABA type A receptor associated protein like 1
- Synonyms gene name:GABA(A) receptor-associated protein like 1
- Synonyms:gec1APG8LATG8LATG8B
- Discovery year:1999-09-29
- Entrez gene record:23710
- Pubmed identification:11414770, 11374880
- Classification:Autophagy related
- VEGA ID:OTTHUMG00000168411
- Gene symbolGABARAPL1-AS1, GABARAPL1
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- GABARAPL1 Polyclonal: ATTO 565
- label filter
- ATTO 565
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies