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Rabbit Anti-Human CDC37 Polyclonal: APC

  • Catalog number
    SPC-142D-APC
  • Product name
    Rabbit Anti-Human CDC37 Polyclonal: APC
  • Size
    100 µg
  • Label
    APC
  • Immunogen
    Native human Cdc37, full length
  • Antibody s target
    Human CDC37
  • Antibody s full description
    Rabbit Anti-Human CDC37 Polyclonal
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Epigenetics
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Hsp90 co chaperone Cdc37 antibody, CDC 37 antibody, Cdc37 antibody, CDC37 cell division cycle 37 homolog antibody, CDC37 cell division cycle 37 S cerevisiae homolog antibody, CDC37 cell division cycle 37 S cerevisiae homolog of antibody, Cdc37 homolog antibody, CDC37 protein antibody, CDC37_HUMAN antibody, CDC37A antibody, cell division cycle 37 antibody, Cell division cycle 37 homolog antibody, Hsp90 chaperone protein kinase targeting subunit antibody, Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody, Hsp90 chaperone protein kinase-targeting subunit antibody, Hsp90 co-chaperone Cdc37 antibody, p50 antibody, p50Cdc37 antibody, S cerevisiae hypothetical protein CDC37 antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1.68 mg/ml
  • Antibody s specificity
    Detects ~44.5kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    HSP90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with HSP90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing HSP90 to its target kinases (1, 2). CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4).
  • Bibliography
    1. Dai K, Kobayashi R., Beach D. (1996) J Biol Chem. 271(36): 22030-22034. 2. Stepanova L, Leng X., Parker S.B., Harper J.W. (1996) Genes Dev. 10(12): 1491-1502. 3. Kimura Y., et al. (1997) Genes Dev. 11(14): 1775-1185. 4. Gray P.J., et al. (2008) Nat Rev Cancer. 8(7): 491-495.
  • Released date
    1/Jan/2012
  • NCBI number
    NP_008996.1
  • Gene number
    11140
  • Protein number
    Q16543
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142) at 1:200 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Human HeLa cell lysates showing detection of CDC37 protein using Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142). Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142) at 1:2000. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody (SPC-142) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-CDC37 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-142) | Rabbit Anti-CDC37 Antibody used in Western blot (WB) on Human HeLa cell lysates (SPC-142) | Rabbit Anti-CDC37 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-142)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "cell division cycle 37 homolog (S. cerevisiae)", "P50CDC37", "CDC37 and IDBG-27267 and ENSG00000105401 and 11140", "Hsp90 protein binding", "Cytoplasm", "Cdc37 and IDBG-139272 and ENSMUSG00000019471 and 12539", "CDC37 and IDBG-643270 and ENSBTAG00000011699 and" ]
    • [ "adenomatous polyposis coli", "BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46", "APC and IDBG-37007 and ENSG00000134982 and 324", "microtubule plus-end binding", "nuclei", "Apc and IDBG-134573 and ENSMUSG00000005871 and 11789", "BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233" ]
  • Gene target
    CDC37 APC
    • Gene info
    Gene info
    Locus Specific Databases:Adenomatous polyposis coli mutation databaseUMD Locus Specific DatabasesGlobal Variome shared LOVDAPC database at LOVD-ChinaLOVD - Leiden Open Variation DatabaseLRG_130
  • Gene symbol
    CDC37, APC
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- CDC37 Polyclonal: APC
  • label filter
    • APC
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies