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Rabbit Anti-Human BIM/BOD (IN2) Polyclonal: ATTO 488

  • Catalog number
    SPC-164D-A488
  • Product name
    Rabbit Anti-Human BIM/BOD (IN2) Polyclonal: ATTO 488
  • Size
    100 µg
  • Label
    ATTO 488
  • Immunogen
    Internal central amino acids of human Bim
  • Antibody s target
    Human BIM
  • Antibody s full description
    Rabbit Anti-Human BIM/BOD (IN2) Polyclonal
  • Primary research fields
    Cancer, Apoptosis
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    BCL2 like protein 11 Antibody, Bcl2 interacting mediator of cell death Antibody, Bcl2 interacting protein Bim Antibody, BCL@ like 11 Antibody, BAM Antibody, BimEl Antibody, BimL Antibody, BOD Antibody, BIM beta 6 Antibody, BIM Beta7 Antibody, BIM alpha 3 Antibody, BIM alpha4 Antibody, BIM alpha5 Antibody, BIM alpha6 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:400), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~23kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    2.5 µg/ml of SPC-164 was sufficient for detection of Bim in 20 µg of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion, Endomembrane System
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
  • Bibliography
    1. O'Connor L., Strasser A., O'Reilly L.A., et al. (1998) EMBO J. 17:384-395. 2. Hsu S.Y., Lin P., and Hsueh A.J. (1998) Mol. Endocrinol. 12:1432-40.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_619527.1
  • Gene number
    10018
  • Protein number
    O43521
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:100. | Western blot analysis of Human A549 cell lysates showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-BIM Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human K562 cells (SPC-164) | Rabbit Anti-BIM Antibody used in Western blot (WB) on Human A549 cell lysates (SPC-164)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    BIM/BOD IN2 ATTO 488
    • Gene info
    Locus Specific Databases:LRG_620
  • Gene symbol
    BCL2L11
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- BIM/BOD (IN2) Polyclonal: ATTO 488
  • label filter
    • ATTO 488
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies