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Rabbit Anti-Human BIM/BOD (IN) Polyclonal: ATTO 488

  • Catalog number
    SPC-113D-A488
  • Product name
    Rabbit Anti-Human BIM/BOD (IN) Polyclonal: ATTO 488
  • Size
    100 µg
  • Label
    ATTO 488
  • Immunogen
    Internal central amino acids of human Bim
  • Antibody s target
    Human BIM/BOD
  • Antibody s full description
    Rabbit Anti-Human BIM/BOD (IN) Polyclonal
  • Primary research fields
    Cancer, Apoptosis
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    BCL2 like protein 11 Antibody, Bcl2 interacting mediator of cell death Antibody, Bcl2 interacting protein Bim Antibody, BCL2 like 11 Antibody, BAM Antibody, BimEl Antibody, BimL Antibody, BOD Antibody, BIM beta 6 Antibody, BIM Beta7 Antibody, BIM alpha 3 Antibody, BIM alpha4 Antibody, BIM alpha5 Antibody, BIM alpha6 Antibody
  • Verified applications
    WB, IHC
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~23kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1 µg/ml of SPC-113 was sufficient for detection of Bim in 20 µg of K562 and A549 cell lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Mitochondrion, Endomembrane System
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1 ,2).
  • Bibliography
    1. O'Connor L., Strasser A., O'Reilly L.A., et al. (1998) EMBO J. 17: 384-395. 2. Hsu S.Y., Lin P., and Hsueh A.J. (1998) Mol. Endocrinol. 12: 1432-40.
  • Released date
    1/Jun/2007
  • NCBI number
    NP_619527.1
  • Gene number
    10018
  • Protein number
    O43521
  • PubMed number
    27689871
  • Tested applications
    Western Blot
  • Tested reactivity
    Human
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-113). Tissue: skin cancer cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-113) at 1:100. | Western blot analysis of Human K562 (Left) and A549 (Right) whole cell lysates showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-113). Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-113) at 1:1000. Rabbit Anti-BIM Antibody used in Immunohistochemistry (IHC) on Human skin cancer cells (SPC-113) | Rabbit Anti-BIM Antibody used in Western blot (WB) on Human K562 (Left) and A549 (Right) whole cell lysates (SPC-113)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    BIM/BOD IN ATTO 488
    • Gene info
    Locus Specific Databases:LRG_620
  • Gene symbol
    BCL2L11
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- BIM/BOD (IN) Polyclonal: ATTO 488
  • label filter
    • ATTO 488
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies