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Rabbit Anti-Human ATG7 Polyclonal: ATTO 700

  • Catalog number
    SPC-609D-A700
  • Product name
    Rabbit Anti-Human ATG7 Polyclonal: ATTO 700
  • Size
    100 µg
  • Label
    ATTO 700
  • Immunogen
    Synthetic peptide from the C-terminal of Human ATG7
  • Antibody s target
    Human ATG7
  • Antibody s full description
    Rabbit Anti-Human ATG7 Polyclonal
  • Primary research fields
    Cancer, Apoptosis, Autophagy, Cardiovascular System, Heart
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Autophagy 7S. cerevisiae, homolog of Antibody, APG7 like Antibody, ATG7_HUMAN Antibody, hAGP7 Antibody, Ubiquitin-like modifier-activating enzyme ATG7 Antibody, Autophagy related protein 7 Antibody, Autophagy-related protein 7 Antibody, GSA 7 Antibody, APG7, S. cerevisiae, homolog of Antibody, Ubiquitin-activating enzyme E1-like protein Antibody, Autophagy-related 7 (yeast) Antibody, APG7 autophagy 7 like Antibody, 1810013K23Rik Antibody, APG7 autophagy 7-like (S. cerevisiae) Antibody, ATG12-activating enzyme E1 ATG7 Antibody, ATG7 autophagy related 7 homolog Antibody, Atg7l Antibody, Ubiquitin activating enzyme E1 like protein Antibody, Apg 7 Antibody, APG7L Antibody, DKFZp434N0735 Antibody, ATG 7 Antibody, APG7-like Antibody, GSA7 Antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) Antibody, ATG7 Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects 77.9 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-609 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Preautophagosomal Structure
  • Tissue specificity
    Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
  • Scientific context
    ATG7 in conjuction with ATG10, mediates the formation of the autophagosome when ATG12 is covalently bound to ATG5 and targets to autophagosome vesicles. It also activates ATG8, and is crucial for amino acid supply in neonates.
  • Bibliography
    1. Mizushima N., et al. (1998) J Biol Chem. 273: 33889-92. 2. Mizushima N., et al. (1998) Nature. 395: 395-8. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-81. 4. Tanida I., et al. (1999) Mol Biol Cell. 10: 1367-79. 5. Shintani T., et al. (1999) EMBO J. 18: 5234-41.
  • Released date
    3/Jan/2017
  • NCBI number
    NP_001129503.2
  • Gene number
    10533
  • Protein number
    O95352
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western blot analysis of Human 293Trap cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody (SPC-609). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (SPC-609) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa. | Western blot analysis of Mouse and rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody (SPC-609). Lane 1: Molecular Weight Ladder. Lane 2: Mouse brain cell lysates. Lane 3: Rat brain cell lysates. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody (SPC-609) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa. Rabbit Anti-ATG7 Antibody used in Western blot (WB) on 293Trap cell lysates (SPC-609) | Rabbit Anti-ATG7 Antibody used in Western blot (WB) on brain cell lysates (SPC-609)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    ATG7 ATTO 700
    • Gene info
  • Gene symbol
    ATG7
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- ATG7 Polyclonal: ATTO 700
  • label filter
    • ATTO 700
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies