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Rabbit Anti-Human ATG5 Polyclonal: ATTO 565

  • Catalog number
    SPC-611D-A565
  • Product name
    Rabbit Anti-Human ATG5 Polyclonal: ATTO 565
  • Size
    100 µg
  • Label
    ATTO 565
  • Immunogen
    Synthetic peptide from the mid-protein of human ATG5
  • Antibody s target
    Human ATG5
  • Antibody s full description
    Rabbit Anti-Human ATG5 Polyclonal
  • Primary research fields
    Cancer, Apoptosis, Cancer, Autophagy, Cardiovascular System, Heart, Cell Signaling, Metabolism, Metabolism processes
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    APG5-like Antibody, ASP Antibody, hAPG5 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-611 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
  • Bibliography
    1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729. 2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.
  • Released date
    1/Oct/2015
  • NCBI number
    NP_001273035.1
  • Gene number
    9474
  • Protein number
     Q9H1Y0
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG5 Antibody (D) Composite. | Western blot analysis of Human HeLa cell lysates showing detection of ~15kDa ATG5 protein using Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG5 Polyclonal Antibody (SPC-611) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~15kDa. Other Band(s): ATG12-ATG5 Complex. Rabbit Anti-ATG5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical Cancer cell line (HeLa) (SPC-611) | Rabbit Anti-ATG5 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-611)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Alternative to gene target
    • [ "autophagy related 5", "APG5 and APG5-LIKE and APG5L and ASP and hAPG5", "ATG5 and IDBG-94856 and ENSG00000057663 and 9474", "protein binding", "Plasma membranes", "Atg5 and IDBG-148568 and ENSMUSG00000038160 and 11793", "ATG5 and IDBG-631895 and ENSBTAG00000005400 and 532686" ]
  • Gene target
    ATG5 ATTO 565
    • Gene info
  • Gene symbol
    ATG5
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- ATG5 Polyclonal: ATTO 565
  • label filter
    • ATTO 565
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies