- Catalog numberSPC-506D-RPE
- Product nameRabbit Anti-Human Amyloid Oligomers (A11) Polyclonal: RPE
- Size100 µl
- PriceAsk For Price
- LabelRPE
- ImmunogenSynthetic molecular mimic of soluble oligomers
- Antibody s targetHuman Amyloid
- Antibody s full descriptionRabbit Anti-Human Amyloid Oligomers (A11) Polyclonal
- Primary research fieldsNeuroscience, Cell Signaling, Cardiovascular System, Blood
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameAmyloid Oligomer alpha beta Antibody, A11 Antibody, Amyloid Oligomer AlphaBeta Antibody, APP Antibody
- Verified applicationsWB, IHC, ICC/IF, IP, ELISA
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat, Eukaryote
- Antibody s recommended dilutions for useWB (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
- Antibody s concentrationSee included datasheet or contact our support service
- Antibody s specificityRecognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysisA 1:1000 dilution of SPC-506 was sufficient for detection of amyloid oligomers in 10 µg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellMembrane
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextAmyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer’s symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson’s disease, and accumulation of polyglutamine-containing aggregates in Huntington’s disease (2, 3).
- Bibliography1. Glabe C.G. (2004) Trends Biochem Sci. 29(10): 542-547. 2. Kayed R., et al. (2004) J Bio. Chem. 279: 46363-46366. 3. Kayed R., et al. (2003) Science. 300(5618): 486-489.
- Released date13/Jan/2012
- NCBI numberNM_000484.2
- Gene number9606
- Protein numberP05067
- PubMed number25862419
- Tested applicationsImmunohistochemistry
- Tested reactivityHuman
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunohistochemistry analysis using Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506). Tissue: Alzheimer's Disease brain. Species: Human. Fixation: Formalin fixed. Primary Antibody: Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506) at 1:1000. Secondary Antibody: Goat Anti-Rabbit ATTO 594 (red). Localization: Plaque. (A) Amyloid Fibril (OC) Antibody (SPC-507). (B) Amyloid Oligomer (A11) Antibody (SPC-506). (C) Composite. Courtesy of: Dr. Elizabeth Head, University of California, Irvine. | Western blot analysis of Human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Oligomers (A11) protein using Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506). Primary Antibody: Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486–489. doi: 10.1126/science.1079469. | Dot blot analysis using Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506). Tissue: Abeta42 fibrils and prefibrillar oligomers. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Oligomers (A11) Polyclonal Antibody (SPC-506) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486–489. doi: 10.1126/science.1079469. Rabbit Anti-Amyloid Oligomers (A11) Antibody used in Immunohistochemistry (IHC) on Human Alzheimer's Disease brain (SPC-506) | Rabbit Anti-Amyloid Oligomers (A11) Antibody used in Western blot (WB) on Human Abeta42 fibrils and prefibrillar oligomers (SPC-506) | Rabbit Anti-Amyloid Oligomers (A11) Antibody used in Dot blot (DB) on Human Abeta42 fibrils and prefibrillar oligomers (SPC-506)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetAmyloid Oligomers A11 RPE
- Identity:HGNC:10293
- Gene:RPE
- Long gene name:ribulose-5-phosphate-3-epimerase
- Discovery year:2001-06-22
- Entrez gene record:6120
- RefSeq identity:NM_006916
- VEGA ID:OTTHUMG00000154690
- Identity:HGNC:34314
- Gene:SNAR-A11
- Long gene name:small NF90 (ILF3) associated RNA A11
- Synonyms gene name:small nuclear ILF3/NF90-associated RNA A11, small ILF3/NF90-associated RNA A11
- Synonyms:snaR-A55323591,
- Discovery year:2008-06-20
- Entrez gene record:100169954
- Pubmed identification:17855395
- RefSeq identity:NR_024225
- Classification:Small NF90 (ILF3) associated RNAs
- Identity:HGNC:8698
- Gene:PCDHGA11
- Long gene name:protocadherin gamma subfamily A, 11
- Synonyms:PCDH-GAMMA-A11
- Discovery year:2000-06-28
- Entrez gene record:56105
- Pubmed identification:10380929
- RefSeq identity:NM_018914
- Classification:Clustered protocadherins
- VEGA ID:OTTHUMG00000164055
- Gene symbolRPE, SNAR-A11, PCDHGA11
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- Amyloid Oligomers (A11) Polyclonal: RPE
- label filter
- RPE
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies