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Rabbit Anti-Human alpha Tubulin Antibody Polyclonal: ATTO 565

  • Catalog number
    SPC-692D-A565
  • Product name
    Rabbit Anti-Human alpha Tubulin Antibody Polyclonal: ATTO 565
  • Size
    100 µg
  • Label
    ATTO 565
  • Immunogen
    Synthetic peptide of Human alpha-Tubulin
  • Antibody s target
    Human alpha Tubulin Antibody
  • Antibody s full description
    Rabbit Anti-Human alpha Tubulin Antibody Polyclonal
  • Primary research fields
    Tags and Cell Markers, Organelle Markers or subcellular markers, Cell Signaling, Cytoskeleton, Microtubules
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Alpha-tubulin ubiquitous Antibody, Alpha tubulin ubiquitous Antibody, Tubulin alpha 1B chain Antibody, Tubulin alpha ubiquitous Antibody, Tubulin K-alpha-1 Antibody, TBA1B_HUMAN Antibody, K alpha 1 Antibody, Tubulin alpha ubiquitous chain Antibody, Tubulin alpha 1B Antibody, TUBA1B Antibody, Tubulin alpha-1B chain Antibody, Tubulin alpha-ubiquitous chain Antibody, Tubulin K alpha 1 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~50 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-692 was sufficient for detection of alpha-Tubulin in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Cytoskeleton
  • Scientific context
    See included datasheet or contact our support service.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_006073.2
  • Gene number
    10376
  • Protein number
    P68363
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha Tubulin Polyclonal Antibody (SPC-692). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-alpha Tubulin Polyclonal Antibody (SPC-692) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) alpha Tubulin Antibody (D) Composite. | Western blot analysis of Human A549 cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa. | Western blot analysis of Human HeLa cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa. Rabbit Anti-alpha Tubulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH 3T3 (Mouse Fibroblast cell line) (SPC-692) | Rabbit Anti-alpha Tubulin Antibody used in Western blot (WB) on A549 cell lysates (SPC-692) | Rabbit Anti-alpha Tubulin Antibody used in Western blot (WB) on HeLa cell lysates (SPC-692)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    alpha Tubulin ATTO 565
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- alpha Tubulin Antibody Polyclonal: ATTO 565
  • label filter
    • ATTO 565
  • technique filter
    • anti-Human
    • Polyclonal
    • Antibody
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, antibodies, rabbit-anti