- Catalog numberSPC-692D-APC
- Product nameRabbit Anti-Human alpha Tubulin Antibody Polyclonal: APC
- Size100 µg
- PriceAsk For Price
- LabelAPC
- ImmunogenSynthetic peptide of Human alpha-Tubulin
- Antibody s targetHuman alpha Tubulin Antibody
- Antibody s full descriptionRabbit Anti-Human alpha Tubulin Antibody Polyclonal
- Primary research fieldsTags and Cell Markers, Organelle Markers or subcellular markers, Cell Signaling, Cytoskeleton, Microtubules
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameAlpha-tubulin ubiquitous Antibody, Alpha tubulin ubiquitous Antibody, Tubulin alpha 1B chain Antibody, Tubulin alpha ubiquitous Antibody, Tubulin K-alpha-1 Antibody, TBA1B_HUMAN Antibody, K alpha 1 Antibody, Tubulin alpha ubiquitous chain Antibody, Tubulin alpha 1B Antibody, TUBA1B Antibody, Tubulin alpha-1B chain Antibody, Tubulin alpha-ubiquitous chain Antibody, Tubulin K alpha 1 Antibody
- Verified applicationsWB, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman
- Antibody s recommended dilutions for useWB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromPeptide Affinity Purified
- Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~50 kDa.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysisA 1:1000 dilution of SPC-692 was sufficient for detection of alpha-Tubulin in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm, Cytoskeleton
- Scientific contextSee included datasheet or contact our support service.
- BibliographyRefer to PubMed
- Released date29/Aug/2016
- NCBI numberNP_006073.2
- Gene number10376
- Protein numberP68363
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha Tubulin Polyclonal Antibody (SPC-692). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-alpha Tubulin Polyclonal Antibody (SPC-692) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) alpha Tubulin Antibody (D) Composite. | Western blot analysis of Human A549 cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa. | Western blot analysis of Human HeLa cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (SPC-692) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa. Rabbit Anti-alpha Tubulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH 3T3 (Mouse Fibroblast cell line) (SPC-692) | Rabbit Anti-alpha Tubulin Antibody used in Western blot (WB) on A549 cell lysates (SPC-692) | Rabbit Anti-alpha Tubulin Antibody used in Western blot (WB) on HeLa cell lysates (SPC-692)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Alternative to gene target
- [ "adenomatous polyposis coli", "BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46", "APC and IDBG-37007 and ENSG00000134982 and 324", "microtubule plus-end binding", "nuclei", "Apc and IDBG-134573 and ENSMUSG00000005871 and 11789", "BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233" ]
- Gene targetalpha Tubulin APC
- Identity:HGNC:583
- Gene:APC
- Long gene name:APC regulator of WNT signaling pathway
- Synonyms gene name:adenomatosis polyposis coli, adenomatous polyposis coli, APC, WNT signaling pathway regulator
- Synonyms:DP2, DP3, DP2.5PPP1R46
- Discovery year:1986-01-01
- Entrez gene record:324
- Pubmed identification:1651563
- RefSeq identity:NM_000038
- Classification:Protein phosphatase 1 regulatory subunits, Armadillo repeat containing
- VEGA ID:OTTHUMG00000128806
- Gene symbolAPC
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- alpha Tubulin Antibody Polyclonal: APC
- label filter
- APC
- technique filter
- anti-Human
- Polyclonal
- Antibody
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, antibodies, rabbit-anti