- Catalog numberSPC-196D-A655
- Product nameRabbit Anti-Human AHA2 Polyclonal: ATTO 655
- Size100 µg
- PriceAsk For Price
- LabelATTO 655
- ImmunogenAha2
- Antibody s targetHuman AHA2
- Antibody s full descriptionRabbit Anti-Human AHA2 Polyclonal
- Primary research fieldsCancer, Heat Shock
- Antibody s categoryPolyclonal Antibodies
- Antibody s other nameATPase hydrogen-exporting ATPase 2 Antibody
- Verified applicationsWB, ICC/IF
- Raised inRabbit
- IsotypeImmunoglobulin
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s recommended dilutions for useWB (1:1000), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
- Antibody s purified fromProtein A Purified
- Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityCross reacts with Aha1.
- Storage recommendations-20°C
- Shipping recommendationsBlue Ice or 4°C
- Certificate of analysis1 µg/ml of SPC-196 was sufficient for detection of Aha2 in 20 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
- Antibody in cellContact our support service.
- Tissue specificitySee included datasheet or contact our support service.
- Scientific contextThe Arabidopsis protein RIN4 is a well known regulator of plant immunity. The plasma membrane H+-ATPases Aha1 and Aha2 are one class of RIN4-associated proteins (1-5). Aha1 and Aha2 play a crucial role in resisting pathogen invasion- plants use RIN4 to regulate H+-ATPase activity during immune responses, thereby controlling stomatal apertures during pathogen attack (1). Wild type AHA2 has been found to be localized to the plasma membrane, and has also been found in the ER (4).
- Bibliography1. Liu J., Elmore J.M., and Coaker G. (2009) Plant Signal Behav. 4(12): 1107-1110. 2. Harper J.F., Surowy T.K. and Sussman M.R. (1989) Proc Natl Acad. Sci USA . 86: 1234-1238. 3. Harper J. F., et al. (1990) J Biol Chem. 265: 13601-13608. 4. Harper J.F., Manney L., and Sussman M.R. (1994) Mol Gen Genet. 243: 572-587. 5. Regenberg B., Villalba J.M., Lanfermeijer F.C., and Palmgren M.G. (1995) Plant Cell. 7: 1655-1666.
- Released date10/Nov/2010
- NCBI numberNP_689605.1
- Gene number130872
- Protein numberQ719I0
- PubMed numberRefer to PubMed
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-AHA2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-196) | Rabbit Anti-AHA2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-196)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilitIn Stock
- Other related products
- Gene targetAHA2 ATTO 655
- HostRabbit, Rabbits
- isotype filter
- Immunoglobulin
- Short nameRabbit Anti- AHA2 Polyclonal: ATTO 655
- label filter
- ATTO 655
- technique filter
- anti-Human
- Polyclonal
- Rabbit
- anti-
- anti
- Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
- Alternative techniquepolyclonals, rabbit-anti, antibodies