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Rabbit Anti-Human AHA2 Polyclonal: ATTO 390

  • Catalog number
    SPC-196D-A390
  • Product name
    Rabbit Anti-Human AHA2 Polyclonal: ATTO 390
  • Size
    100 µg
  • Label
    ATTO 390
  • Immunogen
    Aha2
  • Antibody s target
    Human AHA2
  • Antibody s full description
    Rabbit Anti-Human AHA2 Polyclonal
  • Primary research fields
    Cancer, Heat Shock
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    ATPase hydrogen-exporting ATPase 2 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Cross reacts with Aha1.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1 µg/ml of SPC-196 was sufficient for detection of Aha2 in 20 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
  • Antibody in cell
    Contact our support service.
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    The Arabidopsis protein RIN4 is a well known regulator of plant immunity. The plasma membrane H+-ATPases Aha1 and Aha2 are one class of RIN4-associated proteins (1-5). Aha1 and Aha2 play a crucial role in resisting pathogen invasion- plants use RIN4 to regulate H+-ATPase activity during immune responses, thereby controlling stomatal apertures during pathogen attack (1). Wild type AHA2 has been found to be localized to the plasma membrane, and has also been found in the ER (4).
  • Bibliography
    1. Liu J., Elmore J.M., and Coaker G. (2009) Plant Signal Behav. 4(12): 1107-1110. 2. Harper J.F., Surowy T.K. and Sussman M.R. (1989) Proc Natl Acad. Sci USA . 86: 1234-1238. 3. Harper J. F., et al. (1990) J Biol Chem. 265: 13601-13608. 4. Harper J.F., Manney L., and Sussman M.R. (1994) Mol Gen Genet. 243: 572-587. 5. Regenberg B., Villalba J.M., Lanfermeijer F.C., and Palmgren M.G. (1995) Plant Cell. 7: 1655-1666.
  • Released date
    10/Nov/2010
  • NCBI number
    NP_689605.1
  • Gene number
    130872
  • Protein number
    Q719I0
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody (SPC-196) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-AHA2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-196) | Rabbit Anti-AHA2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-196)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    AHA2 ATTO 390
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- AHA2 Polyclonal: ATTO 390
  • label filter
    • ATTO 390
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies