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Rabbit Anti-Bovine Ubiquitin Polyclonal: ATTO 633

  • Catalog number
    SPC-119B-A633
  • Product name
    Rabbit Anti-Bovine Ubiquitin Polyclonal: ATTO 633
  • Size
    200 µl
  • Label
    ATTO 633
  • Immunogen
    Native bovine Ubiquitin, conjugated to KLH
  • Antibody s target
    Bovine Ubiquitin
  • Antibody s full description
    Rabbit Anti-Bovine Ubiquitin Polyclonal
  • Primary research fields
    Cell Signaling, Post-Translational Modifications, Ubiquitination
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
  • Verified applications
    WB, IHC, ICC/IF, IP
  • Raised in
    Rabbit
  • Isotype
    Immunoglobulin
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Guinea Pig (Cavia porcellus), Pig, Dog, Sheep, Chicken, African clawed frog (Xenopus laevis), Yeast, Fruit Fly (Drosophila melanogaster), Fish, Rainbow Trout (Oncorhynchus mykiss)
  • Antibody s recommended dilutions for use
    WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~10kDa. It also recognizes ubiquinated proteins.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane, Cytoplasm, Nucleus
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
  • Bibliography
    Bibliography
  • Released date
    1/Oct/2008
  • NCBI number
    NP_776558.1
  • Gene number
    281370
  • Protein number
    P0CG53
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human HEK93 lysates showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000. | Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. | Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Rabbit Anti-Ubiquitin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Western blot (WB) on Human HEK93 lysates (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-119) | Rabbit Anti-Ubiquitin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-119)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    - Ubiquitin ATTO 633
  • Host
    Rabbit, Rabbits
  • isotype filter
    • Immunoglobulin
  • Short name
    Rabbit Anti- Ubiquitin Polyclonal: ATTO 633
  • label filter
    • ATTO 633
  • technique filter
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies