gentaur logogentaur logo
x

Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 2A7.H6

  • Catalog number
    SMC-166D-A680
  • Product name
    Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 2A7.H6
  • Size
    100 µg
  • Label
    ATTO 680
  • Clone
    2A7.H6
  • Immunogen
    Full length protein HSP40 (YDJ1)
  • Antibody s full description
    Mouse Anti-Yeast HSP40 Monoclonal IgG1 Kappa Antibody, Clone: 2A7.H6: ATTO 680
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    DnaJ (HSP40) homolog subfmaily B member 1 antibody, DNAJ 1 antibody, DNAJ B1 antibody, DnaJ homolog subfamily B member 1 antibody, DnaJ protein homolog 1 antibody, DNAJ1 antibody, DNAJB 1 antibody, DNAJB1 antibody, DNAJB1 protein antibody, DNJB1_HUMAN antibody, HDJ 1 antibody, HDJ-1 antibody, HDJ1 antibody, Heat shock 40 kDa protein 1 antibody, Heat shock 40kD protein 1 antibody, Heat shock protein 40 antibody, HSP 40 antibody, HSP40 antibody, HSPF 1 antibody, HSPF1 antibody, Human DnaJ protein 1 antibody, Radial spoke 16 homolog B antibody, RSPH16B antibody, Sis1 antibody
  • Raised in
    Mouse
  • Isotype
    IgG1 Kappa, IgG1
  • Antibody s target
    HSP40
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies applications
    WB, IP, ELISA
  • Antibody s reactivity
    Yeast
  • Antibody s dilutions
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~40kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    0.5 µg/ml of SMC-166 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
  • Bibliography
    1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91. 4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890. 7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
  • Released date
    1-Nov-2008
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_014335.1
  • Gene number
    855661
  • Protein number
    P25491
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6 (SMC-166). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-166) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp40 Antibody [2A7.H6] used in Western Blot (WB) on Yeast recombinant cell lysate (SMC-166)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Yeast HSP40 YDJ1 Monoclonal IgG1 Kappa 2A7 H6
    • Gene info
  • Gene symbol
    DNAJB1
  • Host
    mouse, Ascomycota
  • isotype filter
    • IgG1 Kappa
  • Short name
    Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 2A7 H6
  • label filter
    • ATTO 680
  • Clone name
    2A7
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies