- Catalog numberSMC-503D-A700
- Product nameMouse Anti-β-O-Linked N-Acetylgalactosamine Monoclonal IgM, Clone 9B9
- Size100 µg
- PriceAsk For Price
- LabelATTO 700
- Clone9B9
- ImmunogenSynthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Antibody s full descriptionMouse Anti- O-GalNAC Monoclonal IgM Antibody, Clone: 9B9: ATTO 700
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameGalNAC Antibody, O-GalNAC Antibody, β-O-Linked N-Acetylgalactosamine Antibody, O-Linked N-Acetylgalactosamine Antibody, N-Acetylgalactosamine Antibody, 2-(Acetylamino)-2-deoxy-D-galactose Antibody, 2-Acetamido-2-deoxy-D-galactose Antibody, N-Acetylchondrosamine Antibody, 2-Acetamido-2-deoxy-D-galactopyranose Antibody, N-Acetyl-D-galactosamine Antibody, alpha-N-Acetylgalactosamine Antibody, N-Acetylgalactosamine-modified protein Antibody
- Raised inMouse
- IsotypeIgM, IgM
- Antibody s targetO-GalNAC
- Primary research fieldsCell Signaling, Post Translational Modifications, Glycation
- BrandnameMOLECULAR SIGNATURE®
- Antibodies applicationsWB, ICC/IF, ELISA
- Antibody s reactivitySpecies Independent
- Antibody s dilutionsWB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
- PurityPEG Purified
- Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
- Antibody s concentration1 mg/ml
- Antibody s specificitySpecific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysisA 1:1000 dilution of SMC-503 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Antibody in cellSee included datasheet or contact our support service
- Tissue specificitySee included datasheet or contact our support service
- Released date23-Mar-2017
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberRefer to NCBI
- Gene numberRefer to GenBank
- Protein numberRefer to Swiss-Prot
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9 (SMC-503). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody (SMC-503) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of Glycoconjugates showing detection of 67 kDa GalNAc-BSA using Mouse Anti-GalNAc Monoclonal Antibody, Clone 9B9 (SMC-503). Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-GalNAc Monoclonal Antibody (SMC-503) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-O-GalNAc Antibody [9B9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-503) Mouse Anti-O-GalNAc Antibody [9B9] used in Western Blot (WB) on Glycoconjugates (SMC-503)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetβ-O-Linked N-Acetylgalactosamine Monoclonal 9B9
- Hostmouse
- isotype filter
- IgM
- Short nameMouse Anti-β-O-Linked N-Acetylgalactosamine Monoclonal IgM, Clone 9B9
- label filter
- ATTO 700
- Clone name9B9
- technique filter
- anti-
- Mouse
- anti
- IgM
- Techniqueanti-, Mouse, anti, IgM, antibody to, antibodies, IgMs, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies, immunoglobulin-m