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Mouse Anti-β-O-Linked N-Acetylgalactosamine Monoclonal IgM, Clone 9B9

  • Catalog number
    SMC-503D-A390
  • Product name
    Mouse Anti-β-O-Linked N-Acetylgalactosamine Monoclonal IgM, Clone 9B9
  • Size
    100 µg
  • Label
    ATTO 390
  • Clone
    9B9
  • Immunogen
    Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
  • Antibody s full description
    Mouse Anti- O-GalNAC Monoclonal IgM Antibody, Clone: 9B9: ATTO 390
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    GalNAC Antibody, O-GalNAC Antibody, β-O-Linked N-Acetylgalactosamine Antibody, O-Linked N-Acetylgalactosamine Antibody, N-Acetylgalactosamine Antibody, 2-(Acetylamino)-2-deoxy-D-galactose Antibody, 2-Acetamido-2-deoxy-D-galactose Antibody, N-Acetylchondrosamine Antibody, 2-Acetamido-2-deoxy-D-galactopyranose Antibody, N-Acetyl-D-galactosamine Antibody, alpha-N-Acetylgalactosamine Antibody, N-Acetylgalactosamine-modified protein Antibody
  • Raised in
    Mouse
  • Isotype
    IgM, IgM
  • Antibody s target
    O-GalNAC
  • Primary research fields
    Cell Signaling, Post Translational Modifications, Glycation
  • Brandname
    MOLECULAR SIGNATURE®
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    PEG Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-503 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Released date
    23-Mar-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9 (SMC-503). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody (SMC-503) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of Glycoconjugates showing detection of 67 kDa GalNAc-BSA using Mouse Anti-GalNAc Monoclonal Antibody, Clone 9B9 (SMC-503). Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-GalNAc Monoclonal Antibody (SMC-503) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-O-GalNAc Antibody [9B9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-503) Mouse Anti-O-GalNAc Antibody [9B9] used in Western Blot (WB) on Glycoconjugates (SMC-503)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    β-O-Linked N-Acetylgalactosamine Monoclonal 9B9
  • Host
    mouse
  • isotype filter
    • IgM
  • Short name
    Mouse Anti-β-O-Linked N-Acetylgalactosamine Monoclonal IgM, Clone 9B9
  • label filter
    • ATTO 390
  • Clone name
    9B9
  • technique filter
    • anti-
    • Mouse
    • anti
    • IgM
  • Technique
    anti-, Mouse, anti, IgM, antibody to, antibodies, IgMs, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies, immunoglobulin-m