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Mouse Anti-Rat TARP Gamma2/Stargazin Monoclonal IgG2A, Clone S245-1

  • Catalog number
    SMC-421D-A594
  • Product name
    Mouse Anti-Rat TARP Gamma2/Stargazin Monoclonal IgG2A, Clone S245-1
  • Size
    100 µg
  • Label
    ATTO 594
  • Clone
    S245-1
  • Immunogen
    Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2
  • Antibody s full description
    Mouse Anti-Rat Stargazin Monoclonal IgG2A Antibody, Clone: S245-1: ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    CACNG2 Antibody, Calcium channel voltage dependent gamma subunit 2 Antibody, CaV gamma 2 Antibody, Ipr328 Antibody, MGC123981 Antibody, MGC138502 Antibody, MGC138504 Antibody, Voltage-dependent calcium channel gamma-2 subunit Antibody, Neuronal voltage-gated calcium channel gamma-2 subunit Antibody, Stargazin Antibody, Stg Antibody, Stargazer Antibody, TARP Antibody, TARP gamma-2 Antibody, Transmembrane AMPAR regulatory protein gamma-2 Antibody, AW060990 Antibody, B230105C07Rik Antibody, B930041E13Rik Antibody, Wag Antibody, Waggler Antibody
  • Raised in
    Mouse
  • Isotype
    IgG2A, IgG2a
  • Antibody s target
    Stargazin
  • Primary research fields
    Neuroscience, Ion Channels, Calcium Channels, Voltage-Gated Calcium Channels, Cell Structure, Post-Synaptic Markers
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~36kDa. Does not cross-react with TARPGamma3, TARPGamma4 and TARPGamma8.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-421 was sufficient for detection of TARP Gamma2/ Stargazin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
  • Bibliography
    1. Gill M.B., et al. (2011) J Neurosci. 31(18): 6928-6938. 2. Yu L. (2011) Neuroscience. 178: 13-20. 3. Steinmetz C.C., Turrigiano G.G. (2010) J Neurosci. 30: 14685-14690. 4. Kato. A.S. et al. (2007) J Neurosci. 27: 4969-4977. 5. Letts V.A., et al. (1998) Nat Genet. 19: 340-34
  • Released date
    5-Feb-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_445803.1
  • Gene number
    84347
  • Protein number
    Q71RJ2
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TARP Gamma2/Stargazin Monoclonal Antibody, Clone S245-1 (SMC-421). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TARP Gamma2/Stargazin Monoclonal Antibody (SMC-421) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) TARP Gamma2/Stargazin Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of Stargazin Calcium Channel protein using Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody, Clone S245-1 (SMC-421). Primary Antibody: Mouse Anti-Stargazin Calcium Channel Monoclonal Antibody (SMC-421) at 1:100, 1:250, 1:500, and 1:1000. Mouse Anti-TARP Gamma2/Stargazin Antibody [S245-1] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-421) Mouse Anti-Stargazin Calcium Channel Antibody [S245-1] used in Western Blot (WB) on Rat brain lysates (SMC-421)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    TARP Gamma2/Stargazin Monoclonal IgG2A S245-1
    • Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    • Identity:HGNC:37505
    • Gene:PIRC1
    • Long gene name:piwi-interacting RNA cluster 1
    • Discovery year:2009-11-05
    • Entrez gene record:100313844
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
  • Gene symbol
    IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1, PIRC1
  • Host
    mouse
  • isotype filter
    • IgG2A
  • Short name
    Mouse Anti- TARP Gamma2/Stargazin Monoclonal IgG2A, Clone S245-1
  • label filter
    • ATTO 594
  • Clone name
    S245-1
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies