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Mouse Anti-Rat SAP102 Monoclonal IgG1, Clone S19-2

  • Catalog number
    SMC-134D-P594
  • Product name
    Mouse Anti-Rat SAP102 Monoclonal IgG1, Clone S19-2
  • Size
    100 µg
  • Label
    PE/ATTO 594
  • Clone
    S19-2
  • Immunogen
    Fusion protein amino acids 1-120 of rat SAP102
  • Antibody s full description
    Mouse Anti-Rat SAP102 Monoclonal IgG1 Antibody, Clone: S19-2: PE/ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    DLG3 Antibody, MRX90 Antibody, NEDLG Antibody, XLMR Antibody, Disks large homolog 3 Antibody, PSD-95/SAP90-related protein 1 Antibody, Synapse-associated protein 102 Antibody, SAP-102 Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    SAP102
  • Primary research fields
    Neuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Organelle Markers
  • Brandname
    None
  • Antibodies applications
    WB, IHC, IP, ICC/IF, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml was sufficient for detection of Sap102 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
  • Antibody in cell
    Basolateral Cell Membrane , Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Growth Cone
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homology 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
  • Bibliography
    1. Muller B.M., et al. (1996) Neuron 17: 255-265. 2. Lau L. F., et al. (1996) J. Biol. Chem. 271: 21622-21628. 3. Cuthbert P.C., et al. (2007) J Neuroscience 27:2673-2682. 4. Lau L.F., et al. (1996) J Biol Chem 274:5782-5790.
  • Released date
    6-Jul-2009
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_113827.1
  • Gene number
    58948
  • Protein number
    Q62936
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (SMC-134). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (SMC-134) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (SMC-134). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (SMC-134) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SAP102 Antibody [S19-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-134) Mouse Anti-SAP102 Antibody [S19-2] used in Western Blot (WB) on Rat brain lysates (SMC-134)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    SAP102 Monoclonal IgG1 S19-2
    • Gene info
    Locus Specific Databases:Diamond-Blackfan AnemiaLRG_1144
    Gene info
    Locus Specific Databases:Global Variome shared LOVD
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    RPS19, DLG3, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- SAP102 Monoclonal IgG1, Clone S19-2
  • label filter
    • PE ATTO 594
  • Clone name
    S19-2
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies