- Catalog numberSMC-134D-A655
- Product nameMouse Anti-Rat SAP102 Monoclonal IgG1, Clone S19-2
- Size100 µg
- PriceAsk For Price
- LabelATTO 655
- CloneS19-2
- ImmunogenFusion protein amino acids 1-120 of rat SAP102
- Antibody s full descriptionMouse Anti-Rat SAP102 Monoclonal IgG1 Antibody, Clone: S19-2: ATTO 655
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameDLG3 Antibody, MRX90 Antibody, NEDLG Antibody, XLMR Antibody, Disks large homolog 3 Antibody, PSD-95/SAP90-related protein 1 Antibody, Synapse-associated protein 102 Antibody, SAP-102 Antibody
- Raised inMouse
- IsotypeIgG1, IgG1
- Antibody s targetSAP102
- Primary research fieldsNeuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Organelle Markers
- BrandnameNone
- Antibodies applicationsWB, IHC, IP, ICC/IF, AM
- Antibody s reactivityHuman, Mouse, Rat
- Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis1 µg/ml was sufficient for detection of Sap102 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
- Antibody in cellBasolateral Cell Membrane , Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Growth Cone
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextSynapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homology 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
- Bibliography1. Muller B.M., et al. (1996) Neuron 17: 255-265. 2. Lau L. F., et al. (1996) J. Biol. Chem. 271: 21622-21628. 3. Cuthbert P.C., et al. (2007) J Neuroscience 27:2673-2682. 4. Lau L.F., et al. (1996) J Biol Chem 274:5782-5790.
- Released date6-Jul-2009
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberNP_113827.1
- Gene number58948
- Protein numberQ62936
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (SMC-134). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (SMC-134) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SAP102 Antibody (D) Composite. Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 (SMC-134). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody (SMC-134) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SAP102 Antibody [S19-2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-134) Mouse Anti-SAP102 Antibody [S19-2] used in Western Blot (WB) on Rat brain lysates (SMC-134)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetSAP102 Monoclonal IgG1 S19-2
- Identity:HGNC:10402
- Gene:RPS19
- Long gene name:ribosomal protein S19
- Synonyms gene name:loss of heterozygosity on chromosome 19, region 1, loss of heterozygosity, 19, chromosomal region 1
- Synonyms:S19, eS19DBA
- Discovery year:1998-07-22
- Entrez gene record:6223
- Pubmed identification:9582194
- RefSeq identity:NM_001022
- Classification:S ribosomal proteins, MicroRNA protein coding host genes
- VEGA ID:OTTHUMG00000182676
- Identity:HGNC:2902
- Gene:DLG3
- Long gene name:discs large MAGUK scaffold protein 3
- Synonyms gene name:discs, large homolog 3 (neuroendocrine-dlg, Drosophila), discs, large homolog 3 (Drosophila), discs large homolog 3
- Synonyms:NE-Dlg, SAP102, SAP-102NEDLGKIAA1232MRX90PPP1R82
- Discovery year:1997-04-21
- Entrez gene record:1741
- Pubmed identification:9598320
- RefSeq identity:NM_021120
- Classification:Membrane associated guanylate kinases, PDZ domain containing, Protein phosphatase 1 regulatory subunits
- VEGA ID:OTTHUMG00000021778
- Identity:HGNC:31639
- Gene:MIR7-2
- Long gene name:microRNA 7-2
- Synonyms:hsa-mir-7-2
- Discovery year:2004-04-23
- Entrez gene record:407044
- RefSeq identity:NR_029606
- Classification:MicroRNA MIR7 family
- Identity:HGNC:32051
- Gene:MIR329-2
- Long gene name:microRNA 329-2
- Synonyms:hsa-mir-329-2
- Discovery year:2005-07-11
- Entrez gene record:574409
- RefSeq identity:NR_029968
- Classification:MicroRNA MIR329 family
- Identity:HGNC:32091
- Gene:MIR512-2
- Long gene name:microRNA 512-2
- Synonyms:hsa-mir-512-2
- Discovery year:2005-07-15
- Entrez gene record:574459
- RefSeq identity:NR_030181
- Classification:MicroRNA MIR506 family
- Identity:HGNC:32113
- Gene:MIR521-2
- Long gene name:microRNA 521-2
- Synonyms:hsa-mir-521-2
- Discovery year:2005-07-15
- Entrez gene record:574481
- RefSeq identity:NR_030203
- Classification:MicroRNA MIR515 family
- Identity:HGNC:35282
- Gene:MIR1289-2
- Long gene name:microRNA 1289-2
- Synonyms:hsa-mir-1289-2
- Discovery year:2008-11-03
- Entrez gene record:100302134
- RefSeq identity:NR_031621
- Classification:MicroRNA MIR1289 family
- Identity:HGNC:33641
- Gene:MIR509-2
- Long gene name:microRNA 509-2
- Synonyms:hsa-mir-509-2
- Discovery year:2007-10-24
- Entrez gene record:100126301
- RefSeq identity:NR_030586
- Classification:MicroRNA MIR506 family
- Identity:HGNC:18923
- Gene:KRTAP13-2
- Long gene name:keratin associated protein 13-2
- Synonyms:KAP13-2
- Discovery year:2003-02-21
- Entrez gene record:337959
- Pubmed identification:12359730
- Classification:Keratin associated proteins
- VEGA ID:OTTHUMG00000057793
- Gene symbolRPS19, DLG3, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2
- Hostmouse
- isotype filter
- IgG1
- Short nameMouse Anti- SAP102 Monoclonal IgG1, Clone S19-2
- label filter
- ATTO 655
- Clone nameS19-2
- technique filter
- anti-
- Mouse
- anti
- Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies
Gene info
Locus Specific Databases:Diamond-Blackfan AnemiaLRG_1144
Gene info
Locus Specific Databases:Global Variome shared LOVD