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Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa, Clone 13F9

  • Catalog number
    SMC-157D-PCP
  • Product name
    Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa, Clone 13F9
  • Size
    100 µl
  • Label
    PerCP
  • Clone
    13F9
  • Immunogen
    Phosphotyrosine conjugated to KLH
  • Antibody s full description
    Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa Antibody, Clone: 13F9: PerCP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY) Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1 Kappa, IgG1
  • Antibody s target
    Phosphotyrosine
  • Primary research fields
    Cell Signaling, Post-translational Modifications, Phosphorylation
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Ascites
  • Antibody buffer for storage
    Ascites, 0.02% sodium azide
  • Antibody s specificity
    Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
  • Storage recommendations
    4ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
  • Bibliography
    1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.
  • Released date
    1-Apr-2008
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone 13F9 (SMC-157). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-157) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone 13F9 (SMC-157). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-157) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite. Mouse Anti-Phosphotyrosine Antibody [13F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-157) Mouse Anti-Phosphotyrosine Antibody [13F9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-157)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    - Phosphotyrosine Monoclonal IgG1 Kappa 13F9
  • Host
    mouse
  • isotype filter
    • IgG1 Kappa
  • Short name
    Mouse Anti- Phosphotyrosine Monoclonal IgG1 Kappa, Clone 13F9
  • label filter
    • PerCP
  • Clone name
    13F9
  • technique filter
    • Mouse
    • anti
  • Technique
    Mouse, anti, antibody to, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies