- Catalog numberSMC-262D-A655
- Product nameMouse Anti-N-terminal Arginylation Monoclonal IgG3, Clone 2A4
- Size100 µg
- PriceAsk For Price
- LabelATTO 655
- Clone2A4
- ImmunogenSynthetic N-terminal arginylated peptide conjugated to KLH
- Antibody s full descriptionMouse Anti- N-terminal Arginylation Monoclonal IgG3 Antibody, Clone: 2A4: ATTO 655
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameN-terminal Arginine Antibody, N-terminal Arginylation Antibody, N-terminal Arginylated Antibody, N terminal Arginine Antibody, N terminal Arginylation Antibody, N terminal Arginylated Antibody, Amino-terminal Arginine Antibody, Amino-terminal Arginylation Antibody, Amino-terminal Arginylated Antibody, Amino terminal Arginine Antibody, Amino terminal Arginylation Antibody, Amino terminal Arginylated Antibody
- Raised inMouse
- IsotypeIgG3, IgG3
- Antibody s targetN-terminal Arginylation
- Primary research fieldsCell Signaling, Post Translational Modifications, Arginylation
- BrandnameNone
- Antibodies applicationsWB
- Antibody s reactivitySpecies Independent
- Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
- Antibody s concentration1 mg/ml
- Antibody s specificitySpecific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysisA 1:1000 dilution of SMC-262 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellEndoplasmic Reticulum
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextProtein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
- Bibliography1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.
- Released date23-Feb-2017
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberRefer to NCBI
- Gene numberRefer to GenBank
- Protein numberRefer to Swiss-Prot
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendWestern Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 2A4 (SMC-262). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-262) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA and REHKH-BSA. Mouse Anti-N-terminal Arginylation Antibody [2A4] used in Western Blot (WB) on N-terminal Arginine-BSA (SMC-262)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene target- Arginylation Monoclonal IgG3 2A4
- Hostmouse
- isotype filter
- IgG3
- Short nameMouse Anti-N-terminal Arginylation Monoclonal IgG3, Clone 2A4
- label filter
- ATTO 655
- Clone name2A4
- technique filter
- anti-
- Mouse
- anti
- Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies