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Mouse Anti-Human Ubiquitin Monoclonal IgG1, Clone FK2

  • Catalog number
    SMC-214D-PCP
  • Product name
    Mouse Anti-Human Ubiquitin Monoclonal IgG1, Clone FK2
  • Size
    100 µg
  • Label
    PerCP
  • Clone
    FK2
  • Immunogen
    Ubiquitin conjugated lysozyme
  • Antibody s full description
    Mouse Anti-Human Ubiquitin Monoclonal IgG1 Antibody, Clone: FK2: PerCP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    Ubiquitin
  • Primary research fields
    Cell Signaling, Post-translational Modifications, Ubiquitination
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:10000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein A Purified
  • Antibody buffer for storage
    10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide
  • Antibody s concentration
    10 mg/ml
  • Antibody s specificity
    Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
  • Storage recommendations
    4ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
  • Bibliography
    1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.
  • Released date
    12-Jun-2014
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    BAC56955.1
  • Gene number
    7314
  • Protein number
    P0CG47
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. Mouse Anti-Ubiquitin Antibody [FK2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Fibroblast cell line (NIH 3T3) (SMC-214)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Ubiquitin Monoclonal IgG1 FK2
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- Ubiquitin Monoclonal IgG1, Clone FK2
  • label filter
    • PerCP
  • Clone name
    FK2
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies