- Catalog numberSMC-214D-BI
- Product nameMouse Anti-Human Ubiquitin Monoclonal IgG1, Clone FK2
- Size100 µg
- PriceAsk For Price
- LabelBiotin
- CloneFK2
- ImmunogenUbiquitin conjugated lysozyme
- Antibody s full descriptionMouse Anti-Human Ubiquitin Monoclonal IgG1 Antibody, Clone: FK2: Biotin
- Antibody s categoryMonoclonal Antibodies
- Antibody s other namePolyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
- Raised inMouse
- IsotypeIgG1, IgG1
- Antibody s targetUbiquitin
- Primary research fieldsCell Signaling, Post-translational Modifications, Ubiquitination
- BrandnameNone
- Antibodies applicationsWB, IHC, ICC/IF, IP, ELISA
- Antibody s reactivitySpecies Independent
- Antibody s dilutionsWB (1:10000); optimal dilutions for assays should be determined by the user.
- PurityProtein A Purified
- Antibody buffer for storage10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide
- Antibody s concentration10 mg/ml
- Antibody s specificityDetects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
- Storage recommendations4ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysisA 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm , Nucleus
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextUbiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
- Bibliography1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.
- Released date12-Jun-2014
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberBAC56955.1
- Gene number7314
- Protein numberP0CG47
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. Mouse Anti-Ubiquitin Antibody [FK2] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Fibroblast cell line (NIH 3T3) (SMC-214)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetUbiquitin Monoclonal IgG1 FK2
- Hostmouse
- isotype filter
- IgG1
- Short nameMouse Anti- Ubiquitin Monoclonal IgG1, Clone FK2
- label filter
- Biotin
- Clone nameFK2
- technique filter
- anti-Human
- anti-
- Mouse
- anti
- Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies