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Mouse Anti-Human p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12

  • Catalog number
    SMC-152C
  • Product name
    Mouse Anti-Human p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12
  • Size
    25 µg
  • Label
    Unconjugated
  • Clone
    9F12
  • Immunogen
    Full length recombinant protein expressed in E.coli cells
  • Antibody s full description
    Mouse Anti-Human p38 MAPK Monoclonal IgG1 Antibody, Clone: 9F12: Unconjugated
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    p38 MAPK
  • Primary research fields
    Cell Signaling, Post-translational Modifications, Phosphorylation, Cancer
  • Brandname
    None
  • Antibodies applications
    WB, IHC, IP, ELISA, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~38kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
  • Bibliography
    1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001306.1
  • Gene number
    1432
  • Protein number
    Q16539
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute. Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Western Blot analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (SMC-152). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (SMC-152) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-p38 MAPK Antibody [9F12] used in Immunohistochemistry (IHC) on Mouse Retinal Injury Model (SMC-152) Mouse Anti-p38 MAPK Antibody [9F12] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-152) Mouse Anti-p38 MAPK Antibody [9F12] used in Western Blot (WB) on Human Cell lysates (SMC-152)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.025
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    p38 alpha MAP Kinase Monoclonal IgG1 9F12
    • Gene info
    Gene info
    Gene info
    Locus Specific Databases:MASP2base: Mutation registry for MASP-2 deficiencyLRG_82
    Gene info
    Locus Specific Databases:LRG_349
  • Gene symbol
    MOAP1, MAP7, MASP2, MASP1
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- p38 alpha (MAP Kinase) Monoclonal IgG1, Clone 9F12
  • label filter
    • Unconjugated
  • Clone name
    9F12
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies