gentaur logogentaur logo
x

Mouse Anti-Human p23 Monoclonal IgG1, Clone JJ6

  • Catalog number
    SMC-156D-A700
  • Product name
    Mouse Anti-Human p23 Monoclonal IgG1, Clone JJ6
  • Size
    100 µg
  • Label
    ATTO 700
  • Clone
    JJ6
  • Immunogen
    Recombinant human full length p23 protein
  • Antibody s full description
    Mouse Anti-Human p23 Monoclonal IgG1 Antibody, Clone: JJ6: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    co chaperone p23 Antibody, PTGES3 Antibody, TEBP Antibody, telomerase binding protein p23 Antibody, unactive progesterone receptor 23kDa Antibody, HSP90 co-chaperone Antibody, Progesterone receptor complex p23 Antibody, Cytosolic prostaglandin E2 synthase Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    p23
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, ELISA, AM
  • Antibody s reactivity
    Human, Mouse, Rabbit, Chicken, Guinea Pig (Cavia porcellus), Yeast, Yeast (Saccharomyces cerevisiae)
  • Antibody s dilutions
    WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~23kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
  • Bibliography
    1. Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia.
  • Released date
    1-Apr-2008
  • PubMed number
    22806877, 22806877
  • Tested applications
    Immunoprecipitation , Western Blot
  • Tested reactivity
    Human, Human
  • NCBI number
    NP_006592.3
  • Gene number
    10728
  • Protein number
    Q15185
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal, dermal, HF, muscle staining. Bright dermal staining. Western Blot analysis of Human Cell lysates showing detection of p23 protein using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (SMC-156). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (SMC-156) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Uniform epidermal staining – some evidence of cell-cell borders. Mouse Anti-p23 Antibody [JJ6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-156) Mouse Anti-p23 Antibody [JJ6] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-156) Mouse Anti-p23 Antibody [JJ6] used in Western Blot (WB) on Human Cell lysates (SMC-156) Mouse Anti-p23 Antibody [JJ6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-156)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    p23 Monoclonal IgG1 JJ6
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- p23 Monoclonal IgG1, Clone JJ6
  • label filter
    • ATTO 700
  • Clone name
    JJ6
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies