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Mouse Anti-Human HSP70 Monoclonal IgG1, Clone 5A5

  • Catalog number
    SMC-162D-A680
  • Product name
    Mouse Anti-Human HSP70 Monoclonal IgG1, Clone 5A5
  • Size
    100 µg
  • Label
    ATTO 680
  • Clone
    5A5
  • Immunogen
    Human recombinant HSP70 overexpressed in E.coli
  • Antibody s full description
    Mouse Anti-Human HSP70 Monoclonal IgG1 Antibody, Clone: 5A5: ATTO 680
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    HSP70
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, AM
  • Antibody s reactivity
    Human, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Yeast (Saccharomyces cerevisiae), Fruit Fly (Drosophila melanogaster)
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:500), IP (1µg); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.2, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~70kDa. May detect HSP70, HSC70, Grp78 and HSP72.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-162 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Scientific_context
  • Bibliography
    1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
  • Released date
    1-Nov-2008
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_005336.3
  • Gene number
    3303
  • Protein number
    P08107
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 5A5 (SMC-162). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (SMC-162) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. Western Blot analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 (SMC-162). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-162) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-HSP70 Antibody [5A5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical Cancer cell line (HeLa) (SMC-162) Mouse Anti-Hsp70 Antibody [5A5] used in Western Blot (WB) on Rat skeletal muscle tissue lysate (SMC-162)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    HSP70 Monoclonal IgG1 5A5
    • Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    HSPA1B, HSPA1A, HSPA14, HSPA1L
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- HSP70 Monoclonal IgG1, Clone 5A5
  • label filter
    • ATTO 680
  • Clone name
    5A5
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies