gentaur logogentaur logo
x

Mouse Anti-Human HSP70 Monoclonal IgG, Clone C92F3A-5

  • Catalog number
    SMC-100B-A700
  • Product name
    Mouse Anti-Human HSP70 Monoclonal IgG, Clone C92F3A-5
  • Size
    200 µg
  • Label
    ATTO 700
  • Clone
    C92F3A-5
  • Immunogen
    Human HSP70
  • Antibody s full description
    Mouse Anti-Human HSP70 Monoclonal IgG Antibody, Clone: C92F3A-5: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
  • Raised in
    Mouse
  • Isotype
    IgG, IgG
  • Antibody s target
    HSP70
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, ELISA, FCM, FACS, IEM, Bl, AM
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Dog, Chicken, Fruit Fly (Drosophila melanogaster), Carp (Cypriniformes), Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep
  • Antibody s dilutions
    WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~70kDa. Does not cross-react with HSC70 (HSP73).
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Scientific_context
  • Bibliography
    1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424. 8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879. 9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239. 10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377. 11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248. 12. Dressel et al. (2000) J. Immunol. 164: 2362-2371. 13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555. 14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
  • Released date
    1-Jan-2007
  • PubMed number
    28052117, 28053100, 27524398, 27509516, 27527204, 27409630, 27354066, 25552722, 25552722, 25355627, 25412999, 25412999, 25412999, 25034784, 25356750, 25356750, 25200073, 24903326, 25038226, 25400510, 25024384, 24705179, 24625088, 24447452, 24447452, 24516376, 23429211, 23598404, 22763123, 22343013, 22496374, 22050377, 21975426, 22010006, 22039833, 22039833, 21737627, 21320889, 21522149, 21522149, 20924639, 21253609, 21253609, 20130532, 19706692, 19056281, 19209902
  • Tested applications
    Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Flow Cytometry, Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot, Western Blot, Immunoprecipitation, Flow Cytometry, Western Blot, Western Blot, Protein Inhibition, Western Blot, Western Blot, Immunoprecipitation, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Electron Microscopy, Immunoprecipitation , Western Blot, Western Blot, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot
  • Tested reactivity
    Human, Human, Mouse, Human, Human, Human, Human, Human, Human, Human, Bovine, Bovine, Bovine, Human, Mouse, Mouse, Human, Mouse, Mouse, Rat, Human, Rat, Mouse, Human, Human, Mouse, Pig, Human, Rat, Human, Dog, Human , Rat, Pig, Human, Human, Human, Rat, Human, Human, Human, Mouse, Human, Human, Pig, Mouse, Rat
  • NCBI number
    NP_005336.3
  • Gene number
    3303
  • Protein number
    P08107
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (SMC-100) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida. Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Mouse colon carcinoma (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Heat Shocked Melanoma cells (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Western Blot (WB) on Human cell lysates from various cell lines (SMC-100) Mouse Anti-Hsp70 Antibody: FITC [C92] used in Fluorescence Activated Cell Sorting (FACS) on Mouse Heat Shocked CD3+ CD8+ T cells (SMC-100)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    HSP70 Monoclonal C92F3A-5
    • Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    • Identity:HGNC:37527
    • Gene:PIRC23
    • Long gene name:piwi-interacting RNA cluster 23
    • Discovery year:2009-11-05
    • Entrez gene record:100313786
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37526
    • Gene:PIRC22
    • Long gene name:piwi-interacting RNA cluster 22
    • Discovery year:2009-11-05
    • Entrez gene record:100313850
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37524
    • Gene:PIRC20
    • Long gene name:piwi-interacting RNA cluster 20
    • Discovery year:2009-11-05
    • Entrez gene record:100313785
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37525
    • Gene:PIRC21
    • Long gene name:piwi-interacting RNA cluster 21
    • Discovery year:2009-11-05
    • Entrez gene record:100313916
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
  • Gene symbol
    HSPA1B, HSPA14, HSPA1A, HSPA1L, MIR1302-5, PIRC23, PIRC22, PIRC20, PIRC21, HTR6
  • Host
    mouse
  • isotype filter
    • IgG
  • Short name
    Mouse Anti- HSP70 Monoclonal IgG, Clone C92F3A-5
  • label filter
    • ATTO 700
  • Clone name
    C92F3A-5
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
    • IgG
  • Technique
    anti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies, immunoglobulins