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Mouse Anti-Human GRP78 Monoclonal IgG2b, Clone 1H11-1H7

  • Catalog number
    SMC-195D-RPE
  • Product name
    Mouse Anti-Human GRP78 Monoclonal IgG2b, Clone 1H11-1H7
  • Size
    100 µg
  • Label
    RPE
  • Clone
    1H11-1H7
  • Immunogen
    His-tagged human GRP78
  • Antibody s full description
    Mouse Anti-Human GRP78 Monoclonal IgG2b Antibody, Clone: 1H11-1H7: RPE
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regulated Protein 78kDa Antibody, GRP 78 Antibody, GRP-78 Antibody, GRP78_HUMAN Antibody, Heat shock 70 kDa protein 5 Antibody, Heat Shock 70kDa Protein 5 Antibody, HSCe70 Antibody, HSPA 5 Antibody, HSPA5 Antibody, Immunoglobulin Heavy Chain Binding Protein Antibody, Immunoglobulin heavy chain-binding protein Antibody, mBiP Antibody, MIF2 Antibody, Sez7 Antibody
  • Raised in
    Mouse
  • Isotype
    IgG2b, IgG2b
  • Antibody s target
    GRP78
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Fungi, Chestnut Blight (Cryphonectria parasitica), Hamster, Monkey, Rabbit, African clawed frog (Xenopus laevis)
  • Antibody s dilutions
    WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~78kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    0.5 µg/ml of SMC-195 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.
  • Antibody in cell
    Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Lou et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
  • Bibliography
    1. Cho S., et al. (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo S., et al (2006) 26 (15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.
  • Released date
    1-Jul-2011
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001156906.1
  • Gene number
    14828
  • Protein number
    P20029
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 (SMC-195). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-195) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Western Blot analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 (SMC-195). Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-195) at 1:1000. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 (SMC-195). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (SMC-195) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Mouse Anti-GRP78 Antibody [1H11-1H7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-195) Mouse Anti-GRP78 Antibody [1H11-1H7] used in Western Blot (WB) on Human cell lysates (SMC-195) Mouse Anti-GRP78 Antibody [1H11-1H7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-195)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    GRP78 Monoclonal IgG2b 1H11-1H7
    • Gene info
  • Gene symbol
    HSPA5
  • Host
    mouse
  • isotype filter
    • IgG2b
  • Short name
    Mouse Anti- GRP78 Monoclonal IgG2b, Clone 1H11-1H7
  • label filter
    • RPE
  • Clone name
    1H11-1H7
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies